CORE B: IN UTERO ELECTROPORATION CORE

这个核心设施对方案项目的主要好处是它为动物提供了统一的外科手术 而对所有项目的分子操作都需要它们。因此，动物将得到治疗 从他们的母亲到达设施的时间到他们被运往不同的 项目。此外，在这些动物中操纵的神经元也是用同样的 ShRNA及其表达载体的制备。这不仅从解剖学的角度来看很重要 希望将注射大小和位置的个体间差异降至最低 从动物的发育历史来看，这是一个特别重要的因素 正在接受行为测试的动物。活体动物将被项目I和IH使用，并进行灌流 大脑将被削减到项目II。Core已经成功地在子宫电切手术中执行了这些操作 手术和shRNA的设计和使用7年。

The main benefit of this Core facility to the Program Project is that it provides animals with uniform surgical and molecular manipulations to all the Projects tnat require them. Thus, animals will be treated identically from the time of their mothers' arrival to the facility to the time they are shipped to various Projects. In addition, the neurons manipulated in these animals will have been done so with the same preparations of shRNA and expression plasmids. This is important not only from an anatomic perspective¿ where it is hoped that inter-individual variability in injection size and location is minimized¿but also from the point of view of the developmental history of the animal, an especially important factor for animals undergoing behavioral testing. Live animals will be used by Projects I and IH, and perfused brains will be snipped to Project II. The Core has been successfully performing these in utero electtoporations surgeries and shRNA design and use for 7 years.