Monospecific monoclonal antibodies against human transcription factors

(与原件相同) 这是一项建议，旨在创建一个能够实施包括以下内容的管道的生产中心 人转录因子免疫原的获取和鉴定、生产和筛选 单特异性单抗(MMAb)及其生化特性和验证 这些蛋白质捕获(亲和)试剂。 我们强调高吞吐量和可扩展性在我们的 努力生产，并提出开发工艺改进方案，从而提高成本 在获奖期间的质量和质量。我们将保持一定的科学灵活性，以 开发针对较难处理的转录因子蛋白生产亲和试剂的方法 在生产过程中确定的目标。 最后，我们提出了一个符合NIH政策的可行的分配计划，利用现有的 发展中的杂交瘤单克隆库，确保了广泛的可用性和广泛性 在最小限制下便于将来使用试剂的可及性，与实现 这项资助计划的目标。

(same as original) This is a proposal to create a production center capable of implementing a pipeline that includes acquisition and evaluation of human transcription factor immunogens, production and selection of monospecific Monoclonal antibodies (mMAbs), and biochemical characterization and validation of these protein capture (affinity) reagents. We emphasize the advantages of high throughput and scalability to be achieved within our production effort, and propose the development of process improvements, thereby improving cost and quality over the duration of the award. We will maintain a degree of scientific flexibility to develop approaches to producing affinity reagents to less tractable transcription factor protein targets as they are identified in the course of production. Finally we propose a viable distribution plan consistent with NIH policies, utilizing the existing Developmental Hybridoma Monoclonal Bank, which ensures broad availability and wide accessibility for future use of the reagents with minimal constraints, consistent with achieving the goals of this funding initiative.