CORE E: TRANSGENIC CORE

JHU-UMD DRC的转基因和ES细胞核心将使约翰霍普金斯大学的成员 和马里兰州大学合作，以有效地产生转基因和基因敲除小鼠。弗雷德里克·E. Wondisford，医学博士Sally Radovick，M.D.将担任联合主任。两者都是高度 有资格指导这一核心，并直接参与糖尿病和糖尿病相关研究， 进一步加强对JHU-UMD研究者的转基因/基因敲除服务。该中心将利用现有的 约翰霍普金斯大学医学院的转基因核心， 支持以较低的成本加快为DRC成员生成小鼠模型。DRC将 使用高度可靠和高效的方案继续产生靶向ES细胞。智力方面 与转基因和敲除/敲入构建体的产生以及所需的试剂相关 对于许多研究者来说，ES细胞的产生已经被证明是重要的。通过集中经验和 核心设施的专业知识，核心将显着加快中心发现的进展， investigators.转基因服务将位于JHU空间，已经分配给现有的 这是一个设施，这个设施有足够的设备。ES小区服务将位于连接的CMSC中 建筑，毗邻博士. Wondisford的实验室，在500平方米.英尺的空间旺迪斯福德和拉多维克医生 帮助设计靶向结构。核心将由Sidhaye博士管理， 将构建体引入ES细胞，鉴定已经历同源性的ES克隆， 沿着与相应研究者实验室的重组，并制备用于注射入 囊胚促进转基因小鼠从JHU到UMD的运输一直是核心的一部分， 功能另外，核心预算中包含JHU为UMD制造的小鼠的短期住宿费用 成员，如果他们的研究方案需要成像或代谢研究。

The Transgenic and ES Cell Core of the JHU-UMD DRC will enable Members at Johns Hopkins University and the University of Maryland to efficiently generate transgenic and knock-out/in mice. Fredric E. Wondisford, M.D. will serve as Director, and Sally Radovick, M.D. will serve as Co-Director. Both are highly qualified to direct this core and their direct involvement in diabetes and diabetes-related research should further enhance transgenic/knock-out services to JHU-UMD investigators. The Center will utilize the existing Transgenic Core at Johns Hopkins University School of Medicine with the addition of dedicated technical support to expedite generation of mouse models for DRC Members and at a lesser cost. The DRC will continue to generate targeted ES cells using highly reliable and efficient protocols. The intellectual aspects associated with the generation of transgenic and knock-out/knock-in constructs and the reagents necessary for ES cells generation have proved to important for many investigators. By concentrating experience and expertise in this Core facility, the Core will markedly accelerate the progress of discovery for Center investigators. Transgenic services will be located in JHU space that is already allocated to the existing facility, and this facility has adequate equipment. ES cell services will be located in the connected CMSC building, adjacent to Dr. Wondisford's laboratories, in 500 sq. ft. of space. Drs. Wondisford and Radovick will assist in the design of targeting constructs. The Core will be managed by Dr. Sidhaye, who will oversee the introduction of constructs into ES cells, identifying ES clones that have undergone homologous recombination along with the respective investigator's laboratory and preparation of cells for injection into blastocysts. Facilitating transport of genetically altered mice from JHU to UMD has been part of the Core's function. Alternatively, budgeted into the Core'are short-term housing costs at JHU for mice made for UMD Members, if their study protocol requires imaging or metabolic studies.