The Safety of Channel Catfish Pituitary Administered via Intraperitoneal Injectio

项目摘要 使用激素诱导鱼类产卵对许多美国鱼类的成功至关重要。 孵化场/渔业方案。在孵化场的正常产卵作业过程中， 有必要在6 - 8周内每周处理和检查一次鱼。等 这些程序可能对宝贵的亲鱼造成极大的压力，并损害它们的健康。 成功的激素治疗可以减少处理要求，以一个单一的激素管理 随后是配子收集，从而大大减少了整个鱼处理。鲤鱼垂体 (CCP)是最广泛使用的诱导鱼类最终配子成熟（产卵）的药物。信道 还发现鲶鱼（CP）在诱导排卵方面同样有效， 精子形成虽然CP迄今尚未被广泛使用，但它在MS Delta中很容易获得。 美国鲶鱼产业有兴趣获得CP标记，以诱导最终的配子成熟， 癍点叉尾（Ictalurus punctatus），用于生产杂交癍点叉尾（Ictalurus punctatus x 叉尾叉尾带鱼（Ictalurus furcatus）。 拟议项目的具体目标是评估通过以下途径施用CP的安全性 以0 <$、1 <$（12 mg）、2（24 mg）和3（36 mg）CP最大拟定有效剂量/kg鱼体重 （bw）。每组10条鱼将在第1天和第2天注射。在第三天，他们将被剥离产卵，然后 人道地实施安乐死将对蛋的质量进行评估，并通过病理学对鱼进行评估， 组织病理学 该研究将在Thad Cochran国家温水水族馆中心进行 湿实验室（Stoneville MS）。本研究将在此进行，因为该机构（1） （2）有足够资源进行研究，例如： 空间、水、水箱、尸检和组织病理学设施以及管理人员 治疗和收集所需数据）和（3）证明致力于坚持研究 议定书和其他有关准则。 本研究将根据FDA CVM认可的关键靶动物安全性（TAS）进行 研究方案（研究方案编号CP-12-TAS-CCF），旨在向FDA CVM提供 足够的数据来完成TAS部分要求，以批准在癍点叉尾鱼中使用CP。 最终，研究中生成的数据将用于支持补充新兽药 申请使用CP作为雌性癍点叉尾？产卵辅助剂。

Project Summary The use of hormones to induce spawning in fish is critical to the success of many U.S hatchery/fisheries programs. During the course of normal spawning operations at a hatchery, it may be necessary to handle and examine individual fish weekly over a 6 - 8 week period. Such procedures can be extremely stressful to valuable brood stock, and compromise their health. Successful hormone treatment can reduce handling requirements to a single hormone administration followed by gamete collection, thereby greatly reducing overall fish handling. Common carp pituitary (CCP) is the most widely used drug to induce final gamete maturation (spawning) in fish. Channel Catfish Pituitary (CP) has also been found to be similarly effective in inducing ovulation and spermiation. Although CP has not been widely used to date, it is readily available in the MS Delta. The US catfish industry is interested in obtaining a CP label to induce final gamete maturation in channel catfish (Icatlurus punctatus) for the production of hybrid catfish (Ictalurus punctatus x Ictalurus furcatus). The specific objective of the proposed project is to evaluate the safety of CP administered via intraperitoneal (IP) injection to adult female channel catfish (Ictalurus punctatus)/group at 0¿, 1¿ (12 mg), 2¿ (24 mg), and 3¿ (36 mg) the maximum proposed efficacious dose of CP/kg fish body weight (bw). Ten fish per group will be injected on Days 1 and 2. On Day 3 they will be strip spawned then humanely euthanized. The egg quality will be evaluated and the fish evaluated by pathology and histopathology. The study will be conducted at the Thad Cochran National Warmwater Aquaculture Center Wet Lab in Stoneville MS. The study will be conducted here because the facility has (1) a ready accessible source of channel catfish brood stock (2) sufficient resources to conduct the study e.g. space, water, tanks, necropsy and histopathology facilities, as well as personnel to administer treatment and collect required data) and (3) demonstrated commitment to adhere to the study protocol and other relevant guidelines. The study will be conducted under an FDA CVM accepted pivotal Target Animal Safety (TAS) study protocol (Study Protocol Number CP-12-TAS-CCF) and is intended to provide FDA CVM with sufficient data to complete the TAS portion requirements for approval of CP use in channel catfish. Ultimately the data generated in the study will be used to support a supplemental New Animal Drug Application for the use of CP as a spawning aid in female channel catfish.