Genomics & Molecular Engineering Core

DDRCC的基因组和分子工程（GME）核心为遗传物质的分析和操作提供了尖端技术。这些新技术使DDRCC成员能够查明和表征影响消化系统疾病发展和进程的遗传因素，并剖析与消化系统疾病相关的重要途径中的基因功能。GME是一个新的核心，从以前的分子生物学和生物化学核心重组。GME核心分为两个部分。基因型分析组件提供与患者样本的基因分析相关的服务。基因型分析组件的服务包括（1）基于Sequenom Massanray基因分型平台的定制单核苷酸多态性（SNP）基因分型，（2）高兴趣基因的标准SNP基因分型面板，（3）用于基因分型的超高通量DNA序列分析，（4）其他基因型分析方法（如TaqMan），（5）DNA制备，以及（6）统计遗传学支持。 基因工程组件提供与细胞和生物模型系统中基因操作相关的服务。基因工程部分的服务包括（1）利用同源重组敲入或敲除突变的基因的体细胞遗传操作，（2）操纵细菌人工染色体中的大DNA片段的重组工程技术，（3）利用用于异位表达或基因沉默的慢病毒载体构建基因表达构建体，（4）支持实时PCR，支持Odyssey图像分析系统。GME核心通过支持劳动力成本、提供试剂折扣和培训成员掌握新技术来支持成员进行基因型分析和基因工程实验。GME核心的行政总监Nathan Ellis博士和大卫布恩博士监督各组成部分的运作。主任负责确保适当的科学指导和有效利用各部门资源的服务和设施。由于大量的成本节约、相关性以及高质量的服务和资源，GME核心项目的使用率从一开始就迅速增加，预计在本赠款的下一个周期内，使用率将大幅增长。每个组成部分都为不熟悉所支持的实验方法的新的和已建立的研究人员提供培训。全球海洋生态系统核心项目有助于促进多学科合作，并通过共享资源促进富有成效的交流。

The Genomic and Molecular Engineering (GME) Core of the DDRCC provides cutting-edge technologies for the analysis and manipulation of the genetic material. These new technologies empower DDRCC members to pinpoint and characterize genetic factors that influence the development and course of digestive diseases and to dissect gene functions In important pathways relevant to digestive diseases. The GME is a new Core, restructured from the former Molecular Biology and Biochemistry Core. The GME Core divided into two components. The Genotype Analysis component offers sen/ices relating to the genetic analysis of patient samples. The services of the Genotype Analysis component Include (1) customized single nucleotide polymorphism (SNP) genotyping based on the Sequenom Massanray genotyping platfonn, (2) standard SNP genotyping panels for high-Interest genes, (3) ultra-high throughput DNA sequence analysis for genotyping, (4) other genotype analysis methods (such as TaqMan), (5) DNA preparation, and (6) statistical genetics support. The Genetic Engineering component offers services relating to the manipulation of genes in cellular and organismal model systems. The services ofthe Genetic Engineering component include (1) somatic cell genetic manipulation of genes using homologous recombination to knock in or knock out mutations, (2) recombineering technologies to manipulate large DNA segments in bacterial artificial chromosomes, (3) the construction of gene expression constructs using lentiviral vectors for ectopic expression or silencing of genes, (4) support for realtime PCR, and (5) support for the Odyssey image analysis system. The GME Core supports members for genotype analysis and genetic engineering experiments by supporting labor cost, providing discounts for reagents, and training members in new technologies. The Administrative Directors of the GME Core, Drs. Nathan Ellis and David Boone, oversee the operations of the respective components. Directors are responsible for ensuring proper scientific direction and efficient use of services and facilities of the component resources. Usage of the GME Core rapidly increased from start-up because of substantial cost savings, relevance, and high quality of services and resources, and it is anticipated usage will grow substantially during the next cycle of this Grant. Each of the Components offers training of new and established investigators unfamiliar with the supported experimental approaches. The GME Core has helped to foster multidisciplinary collaborations and promote productive exchanges brought about by sharing of resources.