Animal and Microsurgery Core

动物和显微外科核心(核心B)的目的是建立一个重点设施，提供 两个项目中每个项目的调查人员都有足够的动物繁殖给定的基因类型和 为拟议的实验标准化心脏移植和耐受诱导程序。核心B 将提供关键的服务角色，使主要发起人及其工作人员能够专注于 实验和科学方面的努力。对后代进行集中育种和筛选将确保 具有所需基因型别的动物可用于实验。移植物移植由一个集中的 来源将标准化程序，最大限度地减少手术造成的差异，两人使用的移植物 并允许为项目1(研究容忍度的诱导)生成的一些长期接受者 用于项目2(研究公差的维持)，以最大限度地减少实验成本。此外， 麻醉剂和免疫抑制剂等试剂的采购将为两个项目批量进行，以 降低成本。核心B的具体任务将是：a)Ijreed和供两者使用的基因分型动物 项目；b)移植同种异体心脏和进行胸腺摘除手术，由项目负责人和他们的 人员；c)制备和注射捐赠者的脾细胞，并实施指定的免疫抑制疗法；d) 监测移植心脏的跳动情况，移植受者的健康状况，并记录 拒绝；e)按照首席调查员及其工作人员的指示牺牲移植的动物 收集器官用于组织学，并分发给其他项目人员进行表型和功能研究 免疫细胞分析。

The purpose of the Animal and Microsurgery Core (Core B) is to establish a focused facility that provides investigators in each of the two projects with sufficient breeding of animals with given genotypes and with a standardized cardiac transplantation and tolerance induction procedure for the proposed experiments. Core B will provide a key service role that will enable the Principal Invesfigators and their staff to focus on experimental and scientific efforts. Centralized breeding and screening of the progeny will ensure that animals of required genotypes are available for experiments. Transplantafion performed by a centralized source will standardize the procedures, minimize surgically imposed disparities in the grafts used by the two projects and allow some long-term acceptors generated for Project 1 (studying the induction of tolerance) to be used for Project 2 (studying the maintenance of tolerance) to minimize experimental cost. In addifion, purchases of reagents such as anesthetics and immunosuppressants will be done in bulk for both projects to reduce cost. The specific tasks of Core B will be to: a) Ijreed and genotype animals to be used for both projects; b) transplant heart allografts and perform thymectomies as directecj by the project leaders and their personnel; c) prepare and inject donor splenocytes and administer indicated immunosuppressive therapies; d) monitor the beafing of the transplanted hearts, the health of the transplant recipients, and record fimes of rejecfion; e) sacrifice transplanted animals as directed by the Principal Investigators and their personnel and collect organs for histology, as well as to distribute to other project personnel for phenotypic and functional analysis of immune cells.