Neuroanatomy Core

该计划项目的一个基本特征是它在区域、细胞和亚细胞水平上整合了脑干生理学和神经解剖学，因为它与正常和异常的体内平衡有关。核心B，神经解剖学核心，为每个单独的项目提供高质量的、最先进的神经解剖学服务，从而促进计划的综合具体施舍的完成。它是确保计划项目的神经解剖学研究中一致的研究设计和执行的中心资源。它的集中化简化了技术程序，消除了潜在的重复，并促进了项目中神经解剖学方法的整合。该核心还促进了人类神经病理学研究和动物模型之间的比较和交叉受精。神经解剖学核心B将继续设在波士顿儿童医院麻醉学、危重护理和疼痛医学部，并由凯瑟琳·康蒙斯博士领导。她有5-羟色胺神经生物学和神经解剖学的背景，以及尼古丁对5-羟色胺能机制的影响，与小岛屿发展中国家密切相关的对象，以及该计划拟议的具体施舍。工作人员包括一名高度专业和多产的科学家。核心B资源包括康蒙斯博士实验室的显微镜和技术资源，并辅之以科室、科室、医院和整个大学一级的共同资源支持网络。核心B服务包括为个别特定目标提供最佳神经解剖学程序的咨询、方法学方法和技术方面的培训，以及直接参与个别项目的神经解剖学方面。Core B提供的方法包括定量组织受体放射自显影、阵列断层扫描、多重免疫标记、顺行和逆行追踪、联合追踪和免疫标记、轴突终末的定量和成像以及原位杂交。核心B还协助完善研究设计、数据汇总和解释以及手稿准备。到目前为止，核心在神经解剖学方面的贡献已经得到证实，有几份出版物、摘要和手稿正在准备中。这一进展代表着对所有个别项目的贡献。此外，核心B还为跨越一个以上项目的研究做出了贡献，从而促进了该计划项目作为一个整体的积极协作和协同作用。

A cardinal feature of the program project Is Its Integration of brainstem physiology and neuroanatomy at the regional, cellular, and subcellular levels as It relates to normal and abnormal homeostasis. Core B, the Neuroanatomy Core, provides high-quality, state-of-the-art neuroanatomy services to each of the individual projects, thereby facilitating completion of the program's Integrative specific alms. It Is a central resource that ensures consistent study design and execution In the neuroanatomic studies of the program project. Its centralization streamlines technical procedures, eliminates potential duplication, and facilitates Integration of neuroanatomic approaches across the projects. The core also facilitates the comparison and cross- fertilization between studies of human neuropathology and animal models. The Neuroanatomy Core B will continue to be based In the Department of Anesthesiology, Critical Care and Pain Medicine, Children's Hospital Boston, and to be directed by Dr. Kathryn Commons. She has a background in serotonin neurobiology and neuroanatomy, and the effects of nicotine upon serotonergic mechanisms, subjects germane to SIDS and the proposed specific alms of the program. Staffing Includes a highly expert and productive staff scientist. Core B resources Include the microscopy and technical resources of Dr. Commons' laboratory, supplemented by a network of supporting common resources at the division, department, hospital and university-wide level. Core B services Include consultations regarding optimal neuroanatomic procedures for individual specific aims, training In methodological approaches and techniques, and direct participation in the neuroanatomic aspects of Individual projects. Methods available in Core B include quantitative tissue receptor autoradiography, array tomography, multiple Immunolabeling, anterograde and retrograde tract-tracing, combined tract-tracing and Immunolabeling, quantitation and imaging of axonal terminals, and in situ hybridization. Core B also assists with refining study design, data summary and Interpretation and manuscript preparation. The core has a proven track record In neuroanatomic contributions to date In the current cycle, with several resulting publications, abstracts, and manuscripts in preparation. This progress represents a contribution to all of the individual projects. Furthermore, Core B has contributed to studies that span more than one project, thereby contributing to the active collaboration and synergism that characterizes this program project as a whole.