Biological Macromolecule

生物大分子共享资源(BMSR)代表了以前由分子生物学和病毒载体共享资源提供的服务的整合，增加了一项用于重组蛋白生产和纯化的新服务。BMSR的总体目标是促进和实施多个端点，既包括该共享资源内的端点，也包括涉及与其他共享资源接口的端点。这些包括生产研究级病毒颗粒、敲入/敲除小鼠(与转基因/敲除小鼠共享资源合作)、利用同源重组技术敲入/敲除体细胞系、在哺乳动物细胞中瞬时和稳定的蛋白质表达、重组蛋白质的生产(细菌、酵母、昆虫和哺乳动物系统)和用于结构分析的纯化(与结构生物学共享资源合作)，以及使用位于流式细胞术共享资源中的Biacore仪器进行蛋白质相互作用研究。除了将研究人员提供的载体移向这些终点外，我们还为新载体的生成提供设计、构造和验证服务。BMSR由雪莉·泰勒博士(CMG)和Darrell Peterson博士(生物化学和分子生物系)共同指导，前者带来了分子生物学、细胞培养和蛋白质表达经验，后者是蛋白质生产和纯化方面的丰富经验。BMSR雇用了一名资源经理(金伯利·斯特拉顿女士，MS)和两名全职研究专家，他们每人负责三个主要领域中的一个，但在提供的所有服务方面都有足够的交叉培训，以确保连续性和最大效率。 BMSR通常以85%的运力运行，为弗吉尼亚联邦大学(VCU)梅西癌症中心(MCC)和VCU研究社区的广泛调查人员提供服务。2008年1月1日至2010年12月31日期间，获得同行审查资金的管理合作委员会成员约占基本服务和特殊项目的70%。2010年，英国疾病控制与预防中心的病毒媒介服务为五名得到资助的MCC调查员和三名非MCC调查员提供了服务。自2010年1月成立以来，该蛋白质生产设施已经为五名由MCC资助的调查人员和两名非MCC调查人员提供了十个不同项目的服务。BMSR在日常服务和特殊项目中为22名经同行审查资助的MCC调查员和15名非MCC调查员提供服务。

The Biological Macromolecule Shared Resource (BMSR) represents a consolidation of services previously offered by the Molecular Biology and the Virus Vector shared resources, with the addition of a new service for recombinant protein production and purification. The overall goal of the BMSR is to facilitate and implement multiple end-points, both those within this shared resource, and those involving interfacing with other shared resources. These include the production of research grade virus particles, knock-in/knock-out mice (in collaboration with the Transgenic/Knockout Mouse Shared Resource), knock-in/knock-out somatic cell lines using homologous recombination, transient and stable protein expression in mammalian cells, recombinant protein production (bacterial, yeast, insect, and mammalian systems) and purification for structural analysis (in collaboration with the Structural Biology Shared Resource), and for protein interaction studies using the Biacore instrumentation located in the Flow Cytometry shared resource. In addition to moving vectors provided by investigators towards these end points, we offer design, construction and validation services for the generation of new vectors. The BMSR is co-directed by Dr Shirley Taylor (CMG), who brings molecular biology, cell culture, and protein expression experience, and Dr Darrell Peterson (Department of Biochemistry and Molecular Biology) a protein biochemist with extensive experience in protein production and purification. The BMSR employs a Resource Manager (Ms Kimberly Stratton, MS) and two full time research specialists, each of whom takes primary responsibility for one of the three major areas, but with sufficient cross-training in ail services offered to ensure continuity and maximal efficiency. The BMSR generally operates at >85% capacity, serving a wide range of investigators both within the Virginia Commonwealth University (VCU) Massey Cancer Center (MCC), and from the VCU research community. For the period January 1 2008 through December 31, 2010, MCC members with peer-reviewed funding accounted for approximately 70% of basic services and special projects. In CY 2010 the virus vector services of the BMSR served five funded MCC investigators, and three non-MCC investigators. Since its inception in January 2010, the Protein Production facility has served five funded MCC investigators and two non-MCC investigators, on ten separate projects. The BMSR served 22 peer-reviewed funded MCC investigators in routine services and special projects and 15 non-MCC investigators.