Behavioral Core

测量啮齿动物诱发性和自发性疼痛的行为体征是成功的关键。 在这个项目中的所有4个子项目中。行为核心包括8个新设计的考场 研究大楼(MSRBIII)。所有4个项目中的目标1和目标2中的所有行为测试都将进行 由三名训练有素的技术人员盲目操作。所有的行为数据将以电子方式保存，由两个人分析 统计学家，并与项目绩效指标共享。我们将测量诱发的疼痛，如机械性超敏和感冒。 4种临床相关的小鼠疼痛模型中的两性痛觉异常。我们还将使用以下工具测试持续的疼痛 条件性位置偏好和回避(CPP和CPA)。我们还将测量与疼痛相关的共同 发病率，如焦虑和抑郁。我们将检验游泳压力可以延长 疼痛的持续时间。疼痛相关行为将在补充治疗前后进行测试，例如 电针和DHA。该计划项目的中心假设是多重互补 包括电针和天然产物DHA在内的方法可能会改善不同的炎症和 神经炎症过程。我们将在四个临床相关的动物模型中测试我们的假设 以下具体目标：具体目标1：明确神经病理性疼痛、术后疼痛、 4种临床相关小鼠模型的功能性疼痛和三叉神经痛，并测试应激和性别的影响 关于这些模型的疼痛持续时间；具体目标2：确定治疗前和治疗后的效果 电针对神经病理性疼痛、术后疼痛、功能性疼痛和三叉神经痛的电针、AEA、DHA和RvD1的治疗 4个小鼠模型的疼痛；特定目标3(探索性)：为非反射行为制定可操作的行为分析 持续的疼痛。我们将开发和优化使用口腔刺激的口腔面部疼痛的可操作性测量方法。 乌戈·巴西里。我们还将优化发声功能，以检测急性和慢性疼痛。在中测试行为 由同一经验丰富的人员在行为核心建立不同的动物模型并收集数据 使用电子数据报告系统将提高PPG的科学严谨性和可重复性。

The measurement of behavioral signs of evoked and spontaneous pain in rodents is essential to the success of all the 4 subprojects in this program. The Behavioral Core includes 8 newly designed test rooms in a new research Building (MSRBIII). All the behavioral tests in Aim 1 and Aim 2 of all 4 projects will be conducted blindly by three well-trained technicians. All the behavioral data will be saved electronically, analyzed by two statisticians, and shared with Project PIs. We will measure evoked pain such as mechanical allodynia and cold allodynia in both sexes in 4 clinically relevant mouse models of pain. We will also test ongoing pain using conditioned place preference and avoidance (CPP and CPA). We will also measure pain-associated co- morbidity, such as anxiety and depression. We will test the hypothesis that swim stress can prolong the duration of pain. Pain-related behaviors will be tested before and after complementary treatments such as electroacpuncture and DHA. The central hypothesis of this Program Project is that multiple complementary approaches, including electroacupuncture and natural product DHA, may modify distinct inflammation and neuroinflammation processes. We will test our hypotheses in four clinically relevant animal models via the following specific aims: Specific Aim 1: Define the time course of neuropathic pain, postoperative pain, functional pain, and trigeminal pain in 4 clinically relevant mouse models and test the effects of stress and sex on the duration of pain in these models; Specific Aim 2: Ascertain the effects of pre-treatment and post- treatment of EA, aEA, DHA, and RvD1 on neuropathic pain, postoperative pain, functional pain, and trigeminal pain in 4 mouse models; Specific Aim 3 (Exploratory): Develop operant behavioral assays for non-reflexed ongoing pain. We will develop and optimize operant measurement of orofacial pain using Orofacial Stimulation of Ugo Basile. We will also optimize vocalization for detecting acute and chronic pain. Testing behavior in different animal models by the same experienced personnel in the Behavioral Core and collecting the data using electronic-data-report-system will enhance the Scientific Rigor and Reproducibility of the PPG.