Functional Testing Core (FTC)

转基因技术在啮齿动物身上的出现产生了大量的模型，这些模型提供了对感觉功能和病理的分子和细胞方面的见解。WUSM充满活力的研究社区包括许多主要对感觉功能和功能障碍感兴趣的研究人员。此外，由于洞察力有时来自用于其他目的的模型，必须鼓励所有调查人员从更广泛的角度处理他们的模型，并且在提出更大的问题时应该尽可能少遇到障碍。RCAVS功能测试核心(FTC)的目标是使研究人员能够使用最先进的方法和设备，以统一的方式评估小动物的听觉、前庭和视觉系统。因此，FTC维护和操作满足前庭、听觉和视觉测试的不同要求的设备，以便为研究核心中心和更大的研究社区的同事提供构思良好的全面功能测试。FTC的具体目标是：1)促进在小鼠和其他小动物模型上进行全面的感觉(内耳和视觉)测试。提供的听觉测试包括听性脑干反应(ABR)阈值、ABR输入/输出分析、ABR波形和潜伏期分析，以及ABR时间和频谱掩蔽曲线。此外，失真产物耳声发射(DPOAE)提供了有关外毛细胞运动功能的信息。前庭测试主要包括通过分析反射光来跟踪眼睛的运动，因为动物受到控制的旋转。最后，我们的经验表明，内耳和视觉病理经常是一致的。我们已经发布了记录闪光视网膜电信号(ERG)的专业知识，并将这种能力纳入了我们的设备和测试制度。所有测试都可以由核心员工完成，或者我们可以培训调查员自己的员工进行测试。2)提供咨询服务，支持正确应用感官测试和报告结果。如有要求，请提供。核心工作人员协助调查人员解释调查结果、统计分析和陈述约定。3)促进RCAVS研究人员和整个WUSM的互动和新的研究努力。研究核心服务在WUSM内联网上做广告，并在不定期的论坛上进行推广。RCAVS引发了几个部门成员之间的合作，并继续促进不同学科的科学家之间的互动。

The advent of transgenic technology in rodents has yielded a goldmine of models offering insights into molecular and cellular aspects of sensory function and pathology. The vibrant research community at WUSM includes many investigators with a primary interest in sensory function and dysfunction. Moreover, because insights sometimes come from models intended for other purposes, all investigators must be encouraged to approach their models from a broad perspective, and should face as few roadblocks as possible in asking larger questions. The goal of the RCAVS Functional Testing Core (FTC) is to impart to investigators the ability to assess the auditory, vestibular, and visual systems of small animals in a uniform way, applying state-of-the-art methods and equipment. Accordingly, the FTC maintains and operates equipment serving the separate requirements of vestibular, auditory, and visual testing to make well conceived, comprehensive functional testing available to our colleagues within the Research Core Center and the larger research community. The Specific Aims of the FTC are: 1) To facilitate comprehensive sensory (inner ear and visual) testing in mouse and other small animal models. Auditory tests offered include auditory brainstem response (ABR) thresholds, ABR input/output analyses, ABR waveform and latency analysis, and ABR temporal and spectral masking profiles. In addition, distortion product otoacoustic emissions'(DPOAEs) provide information about outer hair cell motor function. Vestibular tests principally encompass tracking of eye movements through analysis of reflected light as animals are subjected to controlled rotations. Finally, our experience has shown that inner ear and visual pathology often coincide. We have published expertise in recording of flash electroretinograms (ERGs) and have incorporated this capability into our equipment and testing regime. All tests can either be done by Core staff, or we can train an investigator's own staff for perform tests. 2) To provide consultative services that support proper application of sensory tests and reporting of results. Upon request. Core staff assist investigators in interpretation of findings, statistical analyses, and presentation conventions. 3) To promote interactions and stimulate new research endeavors by RCAVS investigators and throughout WUSM. Research Core services are advertised on the WUSM intranet, and promoted in occasional forums. The RCAVS has sparked collaborations between members of several departments and continues to seed interactions between scientists in diverse disciplines.