An Instrument for Negative Acoustic Contrast Bead-based Cell Sorting and Staining

描述（由申请人提供）：所提出的用于细胞样品的快速分选和染色的仪器将在许多细胞分析和操作中提供方便的工具，包括诊断（例如，用于分离循环肿瘤细胞或循环FEAL细胞）和治疗（例如，造血干细胞移植、基于干细胞的再生医学），其基于从血液或其它组织中分离稀有细胞。虽然该提议集中于简单的细胞分选和染色方案以及通过流式细胞术对细胞的分析，但是这种通用的模块化系统将允许有效收集和有效制备细胞样品以用于各种临床相关分析，例如基因分型/表型测定（例如，肿瘤基因分型）、药物或化学敏感性测定、药物反应性谱、突变分析和高通量基因测序。因此，拟议的仪器将在广泛的医疗应用领域产生潜在影响，并与改善公共卫生具有普遍意义。

DESCRIPTION (provided by applicant): The proposed instrumentation for rapid sorting and staining of cellular samples will provide a convenient tool in a number of cellular analyses and manipulations including diagnoses (e.g., for isolation of circulating tumor cells or circulating feal cells) and therapies (e.g., hematopoietic stem cells transplantation, stem cell based regenerative medicine) based on rare cell isolation from blood or other tissues. While this proposal focuses on simple cell sorting and staining protocols and analysis of cells by multicolor flow cytometry, this versatile, modular system will allow the efficient collection and efficient preparation of cellular samples for a wide variety of clinically relevant analyses such as genotyping/phenotyping assays (e.g., tumor genotyping), drug or chemosensitivity assays, drug reactivity profiles, mutational analyses and high-throughput gene sequencing. The proposed instrumentation will thus have potential impact across a wide cross section of medical applications and general relevance to the improvement of public health.