Core B: Pathology and High-resolution Imaging Core

项目摘要-核心B 病理学和高分辨率成像核心是支持各种研究的有效核心 通过提供从P01中收集的充分表征和高度注释的存档病理样本， 不同的前列腺癌（PC）细胞系，人类和动物模型实验组织，并通过执行 常规和先进的软组织和硬组织的组织学程序。核心还提供临床 人和小鼠的组织切片和免疫组化解释的诊断评价 前列腺组织和PC骨转移。P01使用动物尸检的研究者还可以访问 先进的技术，如透射电子显微镜，图像分析/形态测量和激光 显微解剖可用的技术包括组织学（特殊染色，冷冻，石蜡和塑料包埋 切片）和免疫组织化学。核心的一个关键优势是，它是嵌入在中心， 代谢性骨疾病在伯明翰的亚拉巴马大学，并与 Cedars-Sinai医学中心（CSMC）和西洛杉矶的生物库和转化研究核心 洛杉矶VA医疗中心这种伙伴关系为核心提供了一个独特的地位， P01研究者在PC骨转移的分子和细胞机制研究中的需求， 包括：a）获取新鲜前列腺和骨转移组织：原发性肿瘤、肿瘤相关的 基质、良性上皮、转移性骨标本和临床标本中与肿瘤无关的基质 在使用激光捕获显微镜的直接形态可视化下进行分子分析; B）作为 形态学核心，为临床标本和动物模型提供PC诊断专业知识，冷冻 切片和光镜设备，组织化学，免疫组织化学，原位杂交和 定量组织形态计量学;和c）提供良好表征的存档病理样品，和 组织微阵列（TMAs）用于筛选具有潜在机制重要性的候选分子， 验证细胞培养和动物模型实验数据，发现新的生物标志物，包括高 吞吐量分析正在进行的集中执行程序的开发解放了P01研究者 避免基本工作的重复，使他们能够利用现有资源提高生产力， 实验时间表它还允许调查人员之间的结果进行比较，并保证 技术准备条件不负责观察到的差异。总之，核心B将继续 为P01提供常规和先进的技术准备，并对两者进行专家解释 对来自人类和动物的细胞和组织样本进行诊断和分子分析。标准化 质量控制程序将最终导致更大的技术统一性，并允许直接 项目之间的数据比较。

Project Summary - Core B The Pathology and High Resolution Imaging Core is an effective nucleus that supports the various studies of the P01 by providing well-characterized, and highly annotated archival pathological samples collected from different prostate cancer (PC) cell lines, human and animal model experimental tissue and by performing routine and advanced histological procedures on soft and hard tissues. The Core also provides clinical diagnostic evaluation of histological sections and immunohistochemical interpretation of human and murine prostatic tissue and PC bone metastases. P01 investigators utilizing animal necropsies also have access to sophisticated techniques, such as transmission electron microscopy, image analysis/morphometry and laser microdissection. Techniques available include histology (special stains, frozen, paraffin- and plastic-embedded sections) and immunohistochemistry. A key advantage of the Core is that it is embedded within the Center for Metabolic Bone Disease at the University of Alabama at Birmingham and works in close partnership with the Biobank and Translational Research Core at Cedars-Sinai Medical Center (CSMC) and with the West Los Angeles VA Medical Center. This partnership provides the Core with a unique position to serve the research needs of P01 investigators in their studies of the molecular and cellular mechanisms of PC bone metastasis, including: a) procurement of fresh prostatic and bone metastatic tissue: primary tumor, tumor-related stroma, benign epithelium, metastatic bone specimens, and stroma unrelated to tumor from clinical specimens under direct morphologic visualization using laser capture microscopy for molecular analyses; b) service as the morphology core, providing PC diagnostic expertise for both clinical specimens and animal models, frozen section and light microscopic facilities, histochemistry, immunohistochemistry, in situ hybridization and quantitative histomorphometry; and c) providing well-characterized, archived pathologic samples and tissue microarrays (TMAs) for screening of candidate molecules of potential mechanistic importance, validation of cell culture and animal model experimental data and discovery of novel biomarkers, including high throughput analysis. The ongoing development of centrally performed procedures frees the P01 investigators from duplication of basic work, allowing them greater productivity with available resources and acceleration of experimental timetables. It also allows comparison of results between investigators with assurance that technical preparatory conditions are not responsible for observed differences. In summary, Core B will continue to provide the P01 with routine and advanced technical preparations and expert interpretation of both diagnostic and molecular analysis of cell and tissue samples from humans and animals. The standardization of the quality-controlled procedures will ultimately lead to greater technical uniformity and allow for direct comparisons of data between projects.