Laboratory

脑血管畸形的综合研究 实验室核心(核心C)将为该计划提供基础设施和实验方法。 核心C将作为小动物外科实验室、细胞和分子生物学实验室、成像 分析实验室和转基因动物繁育单位。PPG内体内实验的关键部分 将在核心执行，从而为实验提供质量控制和一致性。 我们的具体目标是：(目标1)为实验室项目提供几个动物模型，包括 可复制的血管发育不良模型，血流诱导的适应性外向动脉重建模型，以及 成熟啮齿类动物脑内人-鼠移植模型(项目2、3和4)；(目标2)继续 改进我们现有的模型并开发使用生长因子操纵的新的动物模型 刺激、遗传背景和血流动力学操作；(目标3)促进人类移植 并作为人类外科标本的临床组织库。 核心C将协助设计实验、撰写实验方案和解决方法论问题 它还将提供一个讨论和解释结果的论坛。

Integrative Study of Brain Vascular Malformations The Laboratory Core (Core C) will provide infrastructure and experimental methodology for the program. Core C will function as a small animal surgical lab, a cellular and molecular biology lab, an imaging analysis lab, and a transgenic animal breeding unit. Key portions of the in vivo experiments within the PPG will be performed in the Core, thus providing quality control and consistency for the experiments. Our Specific Aims are: (Aim 1) Provide several animal models to the laboratory projects, including reproducible vascular dysplasia models, a flow-induced adaptive outward arterial remodeling model, and human-to-mouse transplantation model in the mature rodent brain (Projects 2, 3 and 4); (Aim 2) Continue to refine our current models and develop novel animal models using manipulation of growth factor stimulation, genetic background and hemodynamic manipulation; (Aim 3) Facilitate transfer of human specimens and serve as the clinical tissue bank for human surgical specimens. Core C will assist in designing the experiments, writing experimental proposals, and solving methodological problems; it will also provide a forum for discussing and interpreting results.