Neuroprotection of Dopamine During Microdialysis

描述（由申请人提供）：微透析广泛用于动物模型和人类患者，以监测和评估活的完整大脑的细胞外神经化学。它对行为的神经化学、CNS病理学、药物发现和临床护理的研究做出了重大贡献。尽管微透析具有许多有益的特性， 具有被忽视的缺点，即植入探针会对被分析的组织造成穿透损伤。穿透损伤导致血脑屏障的打开、探针周围组织的缺血以及星形胶质细胞的激活，星形胶质细胞最终将探针轨道吞没在神经胶质瘢痕中。渗透损伤影响微透析实验的结果，即结果反映了受损组织而不是正常组织的神经化学属性。本文提出的研究目标是建立可行性 改善渗透损伤及其对微透析结果的影响，通过联合使用一种强大的抗炎药物，地塞米松，和一种新的神经靶向的电子和自由基清除细胞保护剂。这项研究的结果将对神经科学的几个分支产生持久而重大的影响，这些分支涉及将设备插入脑组织，包括微透析，电化学传感器和神经假体设备，如深部脑刺激器和神经元记录阵列。

DESCRIPTION (provided by applicant): Microdialysis is widely used in animal models and human patients to monitor and assess the extracellular neurochemistry of the living, intact brain. It has made significant contributions to the study of the neurochemistry of behavior, CNS pathology, drug discovery, and clinical care. Despite its many beneficial attributes, microdialysis suffers from an overlooked drawback in that implanting the probes causes a penetration injury to the tissues being analyzed. The penetration injury results in opening of the blood brain barrier, ischemia of the tissue surrounding the probe, and the activation of astrocytes that eventually engulf the probe track in a glial scar. The penetration injury affects the outcome of microdialysis experiments, i.e. the results reflect the neurochemical attributes of the injured, rather than normal, tissues. The goal of the research proposed here is to establish the feasibility of ameliorating the penetration injury and its impact on microdialysis results by the combined use of a powerful anti-inflammatory drug, dexamethasone, and a novel mitochondrially-targeted electron and radical scavenging cytoprotective agent. The outcome of this research will have a lasting and significant impact on the several branches of neuroscience that involve the insertion of devices into brain tissues, including microdialysis, electrochemical sensors, and neuroprosthetic devices such as deep brain stimulators and neuron recordings arrays.