Microdialysis in the rat striatum: effects of 24 h dexamethasone retrodialysis on evoked dopamine release and penetration injury.
Microdialysis in the rat striatum: effects of 24 h dexamethasone retrodialysis on evoked dopamine release and penetration injury.
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DOI：
10.1021/cn500257x
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发表时间：
2015-01-21
影响因子：
5
通讯作者：
Michael AC
中科院分区：
文献类型：
--
作者：
Nesbitt KM;Varner EL;Jaquins-Gerstl A;Michael AC
微透析用于体内神经化学监测的能力是大力加强微透析程序、探针本身和用于透析液样品分析的分析系统的结果。我们的目标是通过将注意力集中在探针植入脑组织时会发生什么来进一步完善微透析。普遍认为会发生一些组织损伤，使得最接近探针的组织从其正常状态被破坏。我们假设，减轻这种破坏将完善微透析。在此，我们表明，地塞米松，抗炎药，灌注液中的添加保护诱发多巴胺反应，通过快速扫描循环伏安法测量后24小时的探针旁边。我们还表明，地塞米松稳定诱发多巴胺反应测量探头出口超过4-24小时植入后的时间间隔。地塞米松的作用归因于其抗炎作用，因为地塞米松对多巴胺末端的两种组织化学标记物酪氨酸羟化酶和多巴胺转运蛋白没有显著影响。使用组织化学测定，我们证实地塞米松的作用被严格限制在直接的，局部附近的探针。
The power of microdialysis for in vivo neurochemical monitoring is a result of intense efforts to enhance microdialysis procedures, the probes themselves, and the analytical systems used for the analysis of dialysate samples. Our goal is to refine microdialysis further by focusing attention on what happens when the probes are implanted into brain tissue. It is broadly acknowledged that some tissue damage occurs, such that the tissue nearest the probes is disrupted from its normal state. We hypothesize that mitigating such disruption would refine microdialysis. Herein, we show that the addition of dexamethasone, an anti-inflammatory drug, to the perfusion fluid protects evoked dopamine responses as measured by fast-scan cyclic voltammetry next to the probes after 24 h. We also show that dexamethasone stabilizes evoked dopamine responses measured at the probe outlet over a 4–24 h postimplantation interval. The effects of dexamethasone are attributable to its anti-inflammatory actions, as dexamethasone had no significant effect on two histochemical markers for dopamine terminals, tyrosine hydroxylase and the dopamine transporter. Using histochemical assays, we confirmed that the actions of dexamethasone are tightly confined to the immediate, local vicinity of the probe.
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影响因子：
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作者：
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通讯作者：
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