Development of Point-of-Care Diagnostics for Norovirus

项目摘要。 诺如病毒是一组无包膜的单链rna病毒，是主要原因。 在美国，每年有2100万例胃肠炎暴发。当前 预防和控制选择有限，没有疫苗或治疗方法可用。抗体- 基于诺沃克病毒的诊断试剂盒灵敏度低，依赖于抗体 使用。快速、准确、即点诊断试剂盒对于检测和隔离感染至关重要 人事部。我们提出了一种利用人工合成的多聚糖作为捕获分子的新方法。在……里面 根据这项提议，一个跨学科团队将为以下患者开发快速的护理点诊断 诺沃克病毒的灵敏检测。在具体目标1中，我们将使用模块化合成策略来 产生组织血型抗原和Lewis抗原的合成类似物，附件 诺沃克病毒的影响因素。在特定目标2中，我们将开发和评估基于葡聚糖的诊断 在特定目标3中使用诺沃克病毒毒株小组的平台，候选诊断平台将 使用来自暴发和零星的临床样本进行并列比较评估 胃肠道疾病的病例。实现这项提案中的具体目标有望 提供快速、强大、关注点、用户友好的诊断，以实现准确而灵敏的检测 在所有人类诺沃克病毒株中。

Project Summary. Noroviruses, a group of nonenveloped single-stranded RNA viruses, are the leading cause of gastroenteritis outbreaks and account for 21 million cases in the US annually. Current prevention and control options are limited with no vaccines or therapeutics available. Antibody- based norovirus diagnostic kits suffer from low sensitivity and are dependent on the antibody used. Rapid, accurate, point of care diagnostic kits are critical to detect and isolate infected personnel. We propose a novel approach by using synthetic glycans as capture molecules. In this proposal, an interdisciplinary team will develop rapid, point of care diagnostics for the sensitive detection of norovirus. In Specific Aim 1, we will use a modular synthetic strategy to generate synthetic analogs of histo-blood group antigens and Lewis antigens, attachment factors for norovirus. In Specific Aim 2, we will develop and evaluate glycan based diagnostic platforms using panels of norovirus strains In Specific Aim 3, candidate diagnostic platforms will be compared side-by-side evaluated using clinical samples from both outbreaks and sporadic cases of gastrointestinal illness. Accomplishing the specific aims in this proposal is expected to yield rapid, robust, point of care, user friendly diagnostics for the precise and sensitive detection of all human norovirus strains.