Shared Resource: Cell-Based Assay Screening Service

核心：基于细胞的检测筛选服务共享资源(细胞和体内生物学小组) 项目总结 癌症的复杂性和异质性要求基于细胞的技术平台能够 研究人员将揭示推动癌症发展的关键基因、途径、相互作用和修饰 以设计出有效和个性化的治疗方法。基于细胞的化验筛选服务(C-BASS) 共享资源为DLDCC成员提供尖端技术的独特组合， 先进的仪器和基因组资源。C-Bass包含单基因的基本元素 对全基因组筛选的分析，包括全基因组短发夹RNA(ShRNA)文库，BIFC标记 (双分子荧光互补)cDNA文库和多个最先进的自动化机器人 用于文库操作、用于表型分析的高通量筛选和数据处理的仪器 屏幕的基础设施。共享资源由Thomas Westbrook博士和Dan Liu博士执导，他们已经 在开发和实施全基因组筛选技术方面拥有丰富的专业知识。这种组合 专业知识和资源的丰富极大地促进了DLDCC研究人员的癌症研究工作。特定的 C-BASS共享资源提供的服务包括(1)执行全基因组或亚基因组 Scale BIFC和RNAi筛选，(2)利用单独的cDNA和shRNA载体，(3)大规模自动化 CDNA库和shRNA文库的操作和制备，(4)哺乳动物细胞的自动转染和 慢病毒生产，(5)使用自动显微镜或流式细胞术的高通量细胞分析， (6)数据分析、存储和管理。在过去的几个月里，C-Bass还增加了基因组- 使用CRISPR/CAS9系统编辑服务。CRISPR/CAS9不断扩展的功能，它 包括基因打靶、基因组修饰和转录调控，前景是巨大的 为DLDCC成员增加价值。通过将这些资源存放在一个具有凝聚力的设施中，C-Bass能够 癌症中心的研究人员使用了各种基因组和遗传平台，这些平台通常成本和 对于个别研究人员来说，劳动力是令人望而却步的，或者根本不可行。

CORE: Cell-Based Assay Screening Services Shared Resource (Cell & in vivo Biology Group) PROJECT SUMMARY The complexity and heterogeneity of cancer requires cell-based technological platforms that enable researchers to uncover key genes, pathways, interactions, and modifications that drive cancer development in order to devise effective and personalized therapeutics. The Cell-Based Assay Screening Service (C-BASS) Shared Resource provides DLDCC members with a unique combination of cutting-edge technologies, advanced instrumentation, and genomic resources. C-BASS houses essential elements for single-gene analyses to whole-genome screens including genome-wide short-hairpin RNA (shRNA) libraries, BiFC-tagged (Bi-molecular Fluorescence Complementation) cDNA libraries and multiple state-of-the-art automated robotic instruments for library manipulation, high-throughput screening for phenotypic analyses, and data processing infrastructure for screens. The Shared Resource is directed Drs. Thomas Westbrook and Dan Liu who have extensive expertise in developing and implementing technologies for genome-wide screens. This combination of expertise and resources greatly facilitates DLDCC investigators in their cancer research efforts. Specific services provided by the C-BASS Shared Resource include (1) performing whole-genome or sub-genome scale BiFC and RNAi screens, (2) utilizing individual cDNA and shRNA vectors, (3) large-scale automated manipulation and preparation of cDNA and shRNA libraries, (4) automated mammalian cell transfection and lentivirus production, (5) high-throughput cell-based assays using automated microscopy or flow cytometry, and (6) data analysis, storage, and management. In the past few months, C-BASS has also added genome- editing services using the CRISPR/Cas9 system. The ever-expanding functionalities of CRISPR/Cas9, which include gene targeting, genome modification, and transcription modulation, promises to be of tremendous added value to DLDCC members. By housing these resources in a single, cohesive facility, C-BASS enables Cancer Center Investigators to employ a variety of genomic and genetic platforms that are often cost- and labor-prohibitive, or simply not feasible for individual researchers.