CORE C: Genome Wide RNAi Screens

CORE C：全基因组 RNAi 筛选

• 批准号: 7759246
• 负责人: Thomas Westbrook
• 金额: $ 19.81万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7759246
• 关键词: Apoptosis; Area; Back; Bacterial DNA; Base Pairing; Biochemistry; Biological Assay; Candidate Disease Gene; Cell Proliferation; Code; Collection; Complement; Complex; Custom; Data; Data Storage and Retrieval; Development; Failure; Flow Cytometry; Fluorescence Microscopy; Foundations; Future; Genes; Genetic; Genetic Screening; Genomics; Human; Human Resources; Individual; Informatics; Laboratories; Libraries; Mental Retardation and Developmental Disabilities Research Centers; Methods; Molecular Biology; Morphology; Mus; Nervous System Physiology; Organelles; Pathway interactions; Phenotype; Process; Proteins; Proteomics; RNA Interference; RNA library; Research; Research Personnel; Research Support; Resources; Robotics; Screening procedure; Small Interfering RNA; Speed; Surveys; System; Technology; Time; Type 1 Spinocerebellar Ataxia; base; cost; data management; design; experience; genome-wide; instrumentation; loss of function; mouse genome; nervous system disorder; new technology; new therapeutic target; plasmid DNA; programs; relating to nervous system; research study; small hairpin RNA; therapeutic target; tool; vector

The Genome-wide RNAi Analysis Core is a newly established resource at BCM facilitated by the generous support of the Kleberg Foundation, BCM, and the Dept. of Biochemistry & Molecular Biology. While this Core became operational in May 2008, its support of the research programs of BCM and BCM-IDDRC investigators can already be seen by successful genetic screens ongoing in the Core. For instance. Dr. Westbrook is currently conducting a genetic screen for modifiers of REST, a master regulator of neural differentiation programs. This screen is designed to identify new pathways in nervous system function and development using the pooled shRNA-barcoding approach described above. In addition, Dr. Zoghbi's laboratory is in the process of performing a genetic screen for potential therapeutic targets in Spinocerebellar Ataxia type 1 (SCA1). This screen, which combines the shRNA library resource, high-throughput robotics, and flow cytometry of the Core, will identify new therapeutic targets for SCA1 and serve as a roadmap for finding treatments in other neurologic disorders. The potential for the Genome-wide RNAi Analysis Core to facilitate the objectives and research plans for numerous BCM-IDDRC investigators is evident from the proposed utilization by projects listed below. To gauge the level of demand for this newly proposed core among currently supported BCM-IDDRC investigators, a poll was taken. Nearly three quarters of investigators expressed enthusiasm for the core. Its availability will allow future BCM-IDDRC investigators to propose future projects that will take advantage of this type of screening facility. For those projects listed below in proposed utilization, there is immediate demand. It is likely based on the survey of BCM-IDDRC investigators who anticipate using the Core that demand will increase in future years. It is likely that additional

全基因组RNAi分析核心是BCM新建立的资源，由慷慨的 Kleberg基金会、BCM和国防部的支持。生物化学与分子生物学专业。而这个核心 2008年5月开始运作，支持BCM和BCM-IDDRC调查人员的研究方案 已经可以从核心正在进行的成功的基因筛查中看到。例如。威斯布鲁克医生是 目前正在进行REST修饰物的遗传筛查，REST是神经分化的主要调节因子 程序。该屏幕旨在识别神经系统功能和发育的新途径 使用上述汇集的shRNA条形码方法。此外，佐格比博士的实验室在 脊髓小脑性共济失调1型患者潜在治疗靶点的遗传筛选过程 (SCA1)。这个屏幕结合了shRNA文库资源、高通量机器人技术和Flow 核心的细胞学，将识别SCA1的新治疗靶点，并作为发现 其他神经疾病的治疗。 全基因组RNAi分析核心促进目标和研究计划的潜力 从以下所列项目的拟议利用情况可以明显看出许多BCM-IDDRC调查人员。至 评估目前得到BCM-IDDRC支持的调查人员对这一新提出的核心的需求水平， 进行了一项民意调查。近四分之三的调查人员表达了对核心的热情。它的可用性将 允许未来的BCM-IDDRC调查人员提出未来将利用这种类型的 筛查设施。对于下列拟议用途中所列的项目，存在即时需求。很有可能 根据对使用核心的BCM-IDDRC调查人员的调查，需求将在 未来几年。很可能会有额外的