Mutant Mouse Core

Mutant Mouse Core将集中力量并提供维持小鼠数量所需的专业知识 具有通过基因打靶和/或转基因插入产生的各种突变以供参与者使用 调查人员。需要具有特定靶向突变和/或转基因的小鼠的研究人员将能够 从核心获得所需数量的适当基因型别。因此，核心负责 对创始和繁殖动物的常规照料，产生实验动物所需的畜牧业， 它们断奶，应用耳标进行识别，并获得相关基因的基因类型(S)。这个 CORE还负责在商业上不容易获得的情况下提供适当的控制。 育种计划将与每个调查员协调进行，以获得适当的数量 所需基因型别的动物。我们将向PPG续签中的调查人员提供以下内容 基因敲除小鼠；血管紧张素受体1型L A“FLOXED”(Atia-FLOXED)-/-，血管紧张素II受体2型 (AT2)-/Y，内皮型一氧化氮合酶(NOS3或eNOS)-/-，a上皮条件性基因敲除 钠通道(ENaC)在大脑皮层集合管中的单独存在和在大脑皮层集合管加连接处 小管(CNT)和心肌细胞特异性敲除前列腺素EP4受体(CS-EP4)-/-。我们 将提供以下转基因小鼠：核因子-KB/荧光素酶(NF-KB-Luc)TG，和鸡卵清蛋白特异性 A和p T细胞受体(OT II)Tg小鼠。此外，核心将提供老鼠，产生的同源 重组，这在PENaC基因中具有功能增益，作为利德尔综合征的小鼠模型。在……里面 此外，核心将提供以下品系，根据需要直接购买作为实验动物 PPG调查员；Ragl-/-、P47-/-、NZBWF1/J和NZW/LacJ。 相关性(请参阅说明)： PPG的突变小鼠核心，核心C将提供转基因小鼠来支持 将在L-IV项目中进行的调查，如上述各节所述。提供这些核心服务 将使研究人员能够专注于他们的实验，而不必关心 小鼠的日常饲养和基因分型。

The Mutant Mouse Core will centralize effort and provide the expertise necessary to maintain stocks of mice with various mutations generated by gene targeting and/or transgene insertion for use by participating investigators. Investigators requiring mice with specific targeted mutations and/or transgenes will be able to obtain the appropriate genotypes in requisite numbers from the Core. The Core is thus responsible for the routine care of founder and breeding animals, the husbandry required to generate the experimental animals, their weaning, application of ear tags for identification, and obtaining genotypes for the relevant gene(s). The Core is also responsible for supplying suitable controls when they are not readily commercially available. Breeding programs will be carried out in coordination with each investigator to obtain the appropriate number of animals of the required genotypes. We will provide the investigators in the PPG renewal the following gene knockout mice; Angiotensin 1 receptor type l a "floxed" (ATIa-floxed) -/-, angiotensin II receptor type 2 (AT2) -/Y, endothelial nitric oxide synthase (Nos3 or eNOS) -/-, conditional knockouts of the a epithelial sodium channel (ENaC) in both the cortical collecting duct (CCD) alone and the CCD plus the connecting tubule (CNT), and cardiac myocyte specific knockout of the prostaglandin EP4 receptor (CS-EP4) -/-. We will provide the following transgenic mice: NF-KB/luciferase (NF-KB-LUC) Tg, and chicken ovalbumin specific a and p T-cell receptor (OT II) Tg mice. In addition the Core will provide mice, generated by homologous recombination, which have a gain-of-function in the PENaC gene as a mouse model of Liddle's syndrome. In addition the Core will provide the following strains, directly purchased as needed as experimental animals to PPG investigators; Ragl -/-, p47-/-, NZBWF1/J, and NZW/LacJ. RELEVANCE (See instructions): The Mutant Mouse Core of the PPG, Core C will provide genetically modified mice to support the investigations to be conducted in Projects l-IV, as described in those sections. Providing these core services will allow the investigators to concentrate on their experiments without having to concern themselves with the day-to-day husbandry and genotyping of mice.