Project 3

项目3。HIV成功的一个标志是序列的极端变异性，最近的深度测序工作正在显着增加可用数据的数量^^？。28)大多数以前的序列分析集中在gpi20序列，因为它与宿主免疫系统直接相互作用。虽然HFV序列的分歧，他们这样做的进化压力下，和序列，因此，一个强大的信息来源，以确定单个氨基酸在功能特性中发挥的作用。HIV序列变异性不仅影响包膜，而且影响所有HIV蛋白，我们建议将重点放在CA上。CA的主要进化限制与其结构完整性、组装和拆卸过程中的动力学特性以及与宿主蛋白质的相互作用位点有关。我们计划利用HIV和SIV的序列数据来进行CA蛋白序列变异性的分析，并探索其对衣壳蛋白的影响。 结构、动力学和功能。

Project 3. A hallmark of HIVs success is rooted in extreme sequence variability, and recent deep sequencing efforts are increasing the amount of available data dramatically^^?. 28) Most previous sequence analysis focused on gpi20 sequences due to its direct interaction with the host immune system. While HFV sequences diverge, they do so under evolutionary pressures, and sequences are, therefore, a formidable source of information to identify the role that individual amino acids play in functional properties. HIV sequence variability impacts not only envelope but all HIV proteins, and we propose to focus on CA. The major evolutionary constraints on CA are associated with its structural integrity, its dynamic properties during assembly and disassembly, and its interaction sites with host proteins. We plan to exploit HIV and SIV sequence data to carry out an analysis of CA protein sequence variability and explore its impact on capsid structure, dynamics, and function.