Targeted Analysis Resource Core

项目总结/摘要 RTI CHEMICAL Hub的目标分析资源核心（TARC）将提供目标测量 生物介质中的内源性和外源性分析物或分析物类别，包括，但不限于， 全血、毛发、血浆、血清、胎盘、母乳、脐带血、羊水、脑窦液， 组织（例如，肾、肌肉、肝、肺、肠、胃、脾、骨、脑、脂肪）、唾液和 排泄物（尿液、粪便），通过CHEMICAL网络或与发展核心合作获得 （DC）。没有一个单一的仪器平台能够描述这种麻烦，所以TARC的科学家们提出， 具有丰富的色谱-质谱联用（液相色谱-质谱）经验 质谱[LC-MS]和气相色谱-质谱[GC-MS]）和微量元素分析 （ICP-MS）和其他直接相关的技术。将利用这些多个仪器平台， 完成对已知影响环境的化学品和物理暴露的有针对性的测量 儿童健康，包括但不限于多环芳烃、氟化化合物、火焰 阻燃剂、农用化学品、内分泌干扰化学品、邻苯二甲酸盐和有毒金属/微量元素。的 TARC还将完成对心理社会压力标志物的有针对性的测量， 随着生活方式的选择（例如，烟草烟雾，娱乐，处方和设计药物，天然产品） 或新出现的环境化合物（例如，水力压裂液、工程纳米材料、化学品 来自人为和自然灾害）、内源性化合物和营养指标。TARC将 通过以一致和连贯的方式提供标准化的分析和数据，支持RTI CHEMICAL Hub 格式，并在研究设计的背景下提供解释结果的专业知识。TARC还将 通过对适当规模的样本进行分析，确保采用最具成本效益的方法 在可能的情况下，使用自动化样品制备和分析程序; 经过适当培训的工作人员，以满足工作说明书中规定的要求; 一个全面的，完善的质量控制（QC）计划，目前正在使用的现有政府 合同，包括对所有汇总数据、原始数据、数字或文本进行100%独立审计。的 TARC将与RTI CHEAR Hub管理团队（MT）协商，并酌情与CHEAR协商 协调中心和CHINESE客户参与研究设计和方法选择。我们将 与开发核心密切合作，为新的目标建立新的数据捕获或分析方法 方法. TARC将与MT保持密切沟通，TARC核心领导人将在 CHEMICAL指导委员会。TARC将确保将数据传输至CHEMICAL Data 储存库和分析科学中心，并将参与设计和进行环试验。

PROJECT SUMMARY/ABSTRACT The Targeted Analysis Resource Core (TARC) of the RTI CHEAR Hub will provide targeted measurements of endogenous and exogenous analytes or analyte classes in biological media, including, but not limited to, whole blood, hair, plasma, serum, placenta, breast milk, cord blood, amniotic fluid, cerebral sinusoidal fluid, tissues (e.g., kidney, muscle, liver, lung, intestines, stomach, spleen, bone, brain, adipose), saliva, and excreta (urine, feces) obtained through the CHEAR Network or in collaboration with the Development Core (DC). No single instrument platform is capable of characterizing the exposome, so proposed TARC scientists have extensive experience with chromatography-coupled mass spectrometry (liquid chromatography-mass spectrometry [LC-MS] and gas chromatography-mass spectrometry [GC-MS]) and trace element analysis (ICP-MS), and other directly relevant techniques. These multiple instrument platforms will be leveraged to complete targeted measurements of environmental chemicals and physical exposures known to affect children's health, including, but not limited to, polyaromatic hydrocarbons, fluorinated compounds, flame retardants, agrochemicals, endocrine disrupting chemicals, phthalates, and toxic metals/trace elements. The TARC will also complete targeted measurements for markers of psycho-social stress, chemicals associated with lifestyle choices (e.g., tobacco smoke, recreational, prescription, and designer drugs, natural products) or emerging environmental compounds (e.g., hydrofracturing fluids, engineered nanomaterials, chemicals from manmade and natural disasters), endogenous compounds, and nutritional indicators. The TARC will support the RTI CHEAR Hub by providing standardized analysis and data in a consistent and coherent format, and providing expertise for interpreting results in the context of study design. The TARC will also ensure that the most cost-effective approach is used by conducting analysis of samples in appropriate size batches and by using automated sample preparation and analysis procedures when possible; by utilizing appropriately trained staff to meet the requirements defined in the statements of work; and by utilizing a comprehensive, well-established quality control (QC) program currently in use for existing Government contracts, which includes a 100% independent audit of all summarized data, raw data, figures, or text. The TARC will consult with the RTI CHEAR Hub Management Team (MT), and, as appropriate, the CHEAR Coordinating Center and CHEAR clients to participate in study design and selection of methods. We will work closely with the Development Core to establish new data capture or analysis methods for new targeted methods. TARC will remain in close communication with the MT, and the TARC Core Leader will serve on the CHEAR Steering Committee. The TARC will ensure that data are transferred to the CHEAR Data Repository and Analysis Science Center and will participate in the design and conduct of ring trials.