Cell Cycle Regulators in Diabetes and Obesity

在这项建议中，我们将使用细胞周期基因位点发生突变的小鼠模型。我们正在通过评估携带几个细胞周期基因突变的小鼠不同代谢器官的功能来研究葡萄糖耐量和能量动态平衡的机制。这些发现揭示了CDKs和TGG-β蛋白在调节能量平衡过程中的重要作用。我们正在使用组织特异性条件性小鼠和转基因小鼠，利用Cre-loxP技术来抑制或激活候选细胞周期蛋白的表达。这些小鼠模型的特点是评估它们的葡萄糖耐量和能量消耗表型。在适当的情况下，我们通过高脂饮食、锻炼和/或冷暴露来挑战小鼠，以进一步了解细胞周期蛋白在能量代谢中的作用。

In this proposal we will use mouse models that have mutations in the Cell cycle gene loci. We are studying mechanisms of glucose tolerance and energy homeostasis by evaluating functions in different metabolic organs in mice harboring mutations in several cell cycle genes. The findings are revealing important role of Cdks and TGG-beta proteins in processes that modulate energy balance. We are employing tissue-specific conditional mice and transgenic mice to abalte or activate the expression of candidate cell cycle proteins using Cre-loxP technology. These mouse models are them charaterized to evalaute their glucose tolerance and energy expenditure phenotypes. Where appropriate we are challenging the mice with high-fat diet, excercise and/or cold exposure to further understand the role of cell cycle protein in energy metabolism.