Gamete Interactions in Caenorhabditis elegans

相关性：受精是一个生物学过程，具有重要的医学、社会 和经济影响。从广泛的研究中，受精事件是已知的， 一些细节。然而，这些事件的分子基础通常仍然存在， 难以捉摸。以前大多数关于受精的工作都依赖于生物化学和免疫学 接近。我们的工作是开创性的应用经典遗传分析， 这个重要的研究领域 原理：许多针对C. elegans 在传统上用于治疗癌症的其他生物体中是不可用的或很难利用的。 研究施肥。C. elegans是一种能力 分离和保持影响精子或卵子而不影响其他细胞的突变体。在此以前， 通过对不育突变体的研究，我们已经确定了一些第一批分子， 在C.优雅我们的目标是获得更好的 了解精子分子（SPE-9、SPE-38、SPE-41/TRP-3和SPE- 45/Izumo）和卵母细胞分子（EGG-1/2）的功能，以确保成功受精。 SPE-45/Izumo基因代表了从蠕虫到人类的保守精子功能。 进一步说，完全了解受精的分子机制是不可能的 没有更完整的分子成分清单。因此，我们的目标是 通过遗传和分子分析鉴定新的受精分子。特别 重点将放在鉴定卵母细胞所需的基因上。 答：这项提案的目的是进一步了解 受精作用在C. elegans通过进行以下实验目的：1）确定 受精缺陷定义的新配子功能基因的分子本质 突变表型2)调查机械连接、功能和施肥 先前确定的配子功能分子的途径。3)进行饱和 利用一种创新的遗传突变体诱变受精突变体 富集配子功能突变体的筛选策略。这项工作将补充 在其他生物体的生育力研究，以及提供深入了解的机制， 细胞间的相互作用和繁殖策略的多样性。

RELEVANCE: Fertilization is biological process with important medical, social and economic implications. From extensive study, the events of fertilization are known in some detail. However, the molecular underpinnings of these events generally remain elusive. Most previous work on fertilization has relied on biochemical and immunological approaches. Our work is groundbreaking in the application of classic gentic analsysis to this vital area of research. RATIONALE: Many of the genetic and molecular tools developed for C. elegans are not available or are very difficult to utilize in other organisms traditionally used for studying fertilization. One of the most significant advantages of C. elegans is the ability to isolate and maintain mutants that affect sperm or eggs and no other cells. Previously, through the study of sterile mutants, we have identified some of the first molecules required for productive gamete interactions in C. elegans. We aim to get a better understanding how sperm molecules (SPE-9, SPE-38, SPE-41/TRP-3 and SPE- 45/Izumo) and oocyte molecules (EGG-1/2) functions to insure successful fertilization. The SPE-45/Izumo gene represents a conserved sperm function from worms to humans. Futher, a full understanding of the molecular mechanisms of fertilization is impossible without a more complete inventory of molecular components. Therefore we aim to identify new fertilization molecules through genetic and molecular analysis. Particular emphasis will be placed on the identification of genes required in oocytes. OBJECTIVES: The goal of this proposal is to further our understanding of fertilization in C. elegans by conducting the following experimental aims: 1) Determine the molecular nature of new gamete function genes defined by a fertilization defective mutant phenotype. 2) Investigate mechanistic connections, functions and fertilization pathways of previously identified gamete function molecules. 3) Conduct a saturation mutagenesis for fertilization mutants taking advantage of an innovative genetic screening strategy that enriches for gamete function mutants. This work will complement fertility studies in other organisms as well as provide insights into the mechanisms of cell-cell interactions and the diversity of reproductive strategies.