Scientific Core 1 (SC1) In Vivo and Imaging Core

摘要 科学核心1 科学核心1（SC 1）提供在整个计划项目中广泛使用的成像能力。 核心执行：a）显微成像; B）体内血栓形成和血管生成模型;和c）分离 用于离体实验的来自小鼠的细胞。显微镜成像是迄今为止该核心的主要服务。这 部分是组织利用现有的仪器和专业知识内勒纳研究 克利夫兰诊所研究所。仪器包括6台Leica DM显微镜、2台Leica DMI 6000 用于延时成像的显微镜，TIRF系统，两台Leica TCS-SP2激光扫描共聚焦显微镜， Leica SCN 400载玻片扫描仪，Leica激光显微切割系统，LI-COR Odyssey，基于激光的扫描 用于凝胶、细胞内蛋白质印迹、ELISA/弗里萨、EMSA/凝胶迁移、蛋白质检测的系统，以及新的FEI Tecnai G2 配备11 MPix Gatan Orius 832 CCD相机的Spirit BioTWIN透射电子显微镜。 除了仪器之外，核心联合主任Judy Drazba博士的真正专业知识， 20年的显微镜经验，对所有仪器的实际操作熟悉程度和培训能力 所有项目的调查人员都能正确使用仪器和解释数据。 该核心的体内部分在小鼠中进行血栓形成、止血和血管生成模型。的 要进行的模型是FeCl 3和玫瑰红诱导的肠系膜和颈动脉血栓形成， 尾部出血以定量止血，基质胶栓塞和RM-1前列腺肿瘤作为血管生成。的部分 由尤金波德雷兹博士领导，他在这些模型的性能方面经验丰富， 在上一个供资期间，类似的和成功的核心。SC 1的第三元件执行以下隔离： 鼠内皮细胞和平滑肌细胞。这些细胞被用于两个项目中， 由有经验的技术人员进行的分离增加了建立这种培养物的成功， 细胞行为的一致性。通过提供熟练的领导和组织，SC 1体现了 通过将关键职能和专业知识集中到计划项目的核心中，可以获得这些优势。

Abstract Scientific Core 1 Scientific Core 1(SC1) provides imaging capabilities that are used broadly throughout the Program Project. The core performs: a) microscopic imaging; b) in vivo thrombosis and angiogenesis models; and c) isolation of cells from mice for ex vivo experiments. Microscopic imaging is by far the major service of this Core. This portion is organized to take advantage of the existing instrumentation and expertise within the Lerner Research Institute of the Cleveland Clinic. The instrumentation includes 6 Leica DM Microscopes, two Leica DMI6000 microscopes for time lapse imaging, a TIRF system, two Leica TCS-SP2 laser scanning confocal microscopes, a Leica SCN 400 slide scanner, a Leica laser microdissection system, LI-COR Odyssey, laser-based scanning system for gels, in-cell Westerns, ELISA/FLISA, EMSA/Gel Shift, Protein Detection, and a new FEI Tecnai G2 Spirit BioTWIN Transmission Electron Microscope equipped with an 11MPix Gatan Orius 832 CCD camera. Beyond the instrumentation is the true expertise of the Core Co-Director, Dr. Judy Drazba, who has more than 20 years of experience in microscopy, hands-on familiarity with all of the instruments and the ability to train investigators from all of the projects in the appropriate use of instrumentation and in the interpretation of data. The in vivo portion of this core performs thrombosis, hemostasis and angiogenesis models in mice. The models to be performed are FeCl3 and rose Bengal induced thrombosis to the mesenteric and carotid arteries, tail bleeding to quantify hemostasis, and matrigel plug and RM-1 prostate tumor as angiogenesis. The portion of the Core is led by Dr. Eugene Podrez, who is experienced in the performance of these models and led a similar and successful core in the previous funding period. The third element of SC1 performs isolation of murine endothelial cells and smooth muscle cells. These cells are used in two of Projects and having these isolations performed by experienced technologists adds to the success of establishing such cultures and the uniformity of behavior of the cells. By providing skilled leadership and organization, SC1 epitomizes the value that can be gained from centralization of key functions and expertise within a Core within the Program Project.