Zeiss LSM710 Inverted Confocal Microscope

 描述（由申请人提供）：特拉华州（UD）的大学有一个非常高的研究活动（卡内基分类），这一活动的大部分集中在生物医学研究的不同领域。特别是艺术与科学学院和工程学院在很大程度上依赖于先进的成像仪器进行组织，细胞和生物材料的研究。UD通过已建立的多用户成像核心设施最大限度地利用了这些仪器，该设施负责维护、安排设备使用、培训用户和就先进技术项目或新成像技术的开发提供咨询。我们在UD获得的第一批共聚焦显微镜之一，LSM 510维斯共聚焦显微镜，位于UD的特拉华州生物技术研究所（DBI）生物成像中心。该仪器现已使用15年，由于图像质量差而不再使用，制造商自2009年9月起不再提供服务合同。该申请是重新提交的申请，要求提供资金，用带环境室的LSM 710倒置共聚焦显微镜更换这台不再满足用户社区需求的过时仪器。拟议的系统将位于DBI的显微镜套件中，并由生物成像中心的技术人员管理。这将确保使用DBI生物成像中心的所有研究人员都能使用拟议的显微镜，该中心是UD众多NIH资助研究项目的关键核心设施。目前，DBI生物成像中心只有LSM510代倒置共聚焦显微镜，缺乏活细胞实验的灵敏度和环境控制。因此，我们需要一台LSM710倒置共聚焦显微镜来支持NIH资助的研究人员研究各种生物医学过程，包括工程血管移植、程序性细胞死亡、心血管、生物力学、唾液腺、声带生物工程、眼睛发育、血栓形成、骨形成和HIV组装。

 DESCRIPTION (provided by applicant): The University of Delaware (UD) has a very high research activity (Carnegie Classification) and much of this activity focuses on diverse fields of biomedical research. In particular the College of Arts and Sciences and the College of Engineering depend heavily on advanced imaging instruments for research in tissues, cells and biomaterials. UD has maximized utilization of these instruments with an established multi-user imaging core facility, which is responsible for maintaining, scheduling equipment usage, training users and consulting on advanced technical projects or development of new imaging techniques. One of the first confocal microscopes we acquired at UD, an LSM510 VIS confocal microscope, is housed in the Delaware Biotechnology Institute (DBI) Bio-Imaging Center at UD. The instrument is now 15 years old and no longer in use due to poor image quality and the manufacturer no longer providing service contracts as of September 2009. This application is a resubmission requesting funds to replace this obsolete instrument, which no longer meets the needs of our user community, with an LSM710 inverted confocal microscope with an environmental chamber. The proposed system will be located in the microscopy suite at the DBI and administered by the Bio- Imaging Center technical staff. This will ensure access to the proposed microscope to all investigators who utilize the DBI Bio-Imaging Center, which is a critical core facility for numerous NIH funded research projects at UD. Currently, the DBI Bio-Imaging Center only has LSM510 generation inverted confocal microscopes that lack the sensitivity and environmental control for live-cell experiments. Therefore, we are requested an LSM710 inverted confocal microscope to support NIH funded investigators researching a diverse array of biomedical processes that include engineering vascular grafts, programmed cell death, cardiovascular, biomechanics, salivary glands, vocal fold bioengineering, eye development, thrombosis, bone formation and HIV assembly.