Chemical Synthesis and High Throughput Analytics Shared Resource

翻译研究小组 核心010 -化学合成和高通量分析共享资源 项目总结/摘要 范德比尔特化学合成和高通量分析共享资源（CSHTASR）提供 范德比尔特-英格拉姆癌症中心（VICC）研究人员利用 化学和全基因组siRNA，以支持需要应用化学工具的研究， 生物问题。由该共享资源支持的项目分为以下几类：1）医学 化学，包括用于临床前或假设驱动的靶向和单个化学探针合成， 体外研究; 2）用于体内动物研究的大规模合成（10-100克）; 3）合成同位素 标记的化合物; 4）亲和和荧光探针; 5）修饰的核苷酸和肽的合成;和 6)高通量分析仪器，为多种实验设计提供读数， 从生物化学到基于图像的表型分析; 7）高达75，000个的全基因组siRNA文库筛选 siRNA; 8）非常多样化的化学文库的低、中和高通量化学筛选。的 CSHTASR通过仪器技术和人员知识的结合提供这些服务。 机构和国家卫生研究院的资金已经投入，以获得有效的 高效的CSHTA服务。此外，CSHTASR的小分子和siRNA文库是有价值的 物质资源，允许正在研究的生物现象的询问，提供洞察力， 目标/途径鉴定和化学探针优化的起始点。CSHTASR采用了 训练有素的领导和支持人员，在先进的化学技术方面拥有丰富的经验， HTS方法。协商方法用于通过共享资源进行的项目;这 是一种必要和有益的模式，可使国际刑事法院调查员和 共享资源的工作人员，以提供最佳的方法来满足每个人的需求。

TRANSLATIONAL RESEARCH GROUP CORE 010 – CHEMICAL SYNTHESIS AND HIGH-THROUGHPUT ANALYTICS SHARED RESOURCE PROJECT SUMMARY/ABSTRACT The Vanderbilt Chemical Synthesis and High-throughput Analytics Shared Resource (CSHTASR) provides state-of-the-art capabilities for Vanderbilt-Ingram Cancer Center (VICC) researchers to harness the power of chemistry and whole genome siRNA to support research requiring the application of chemical tools to answer biological questions. Projects supported by this shared resource fall into the categories of: 1) medicinal chemistry, including hit-to-lead and individual chemical probe synthesis for preclinical or hypothesis-driven in vitro studies; 2) large-scale synthesis (10-100 grams) for in vivo animal studies; 3) synthesis of isotopically labeled compounds; 4) affinity and fluorescent probes; 5) synthesis of modified nucleotides and peptides; and 6) high-throughput analytics instrumentation that provide readouts for multiple experimental designs ranging from biochemical to image-based phenotypic assays; 7) whole genome siRNA library screens of up to 75,000 siRNAs; 8) low-, medium- and high-throughput chemical screens of very diverse chemical libraries. The CSHTASR provides these services through a combination of instrument technology and personnel knowledge. Institutional and NIH funds have been invested to acquire leading-edge instrumentation required for effective and efficient CSHTA services. In addition, the CSHTASR’s small molecule and siRNA libraries are valuable material resources that allow interrogation of the biological phenomena being studied, providing insight into target/pathway identification and starting points for chemical probe optimization. The CSHTASR employs a highly trained leadership and support staff with extensive experience in advanced chemistry techniques and HTS methodologies. A consultative approach is used for projects that come through the shared resource; this is a necessary and beneficial model that allows transfer of knowledge between VICC investigator and the shared resource staff to provide the optimal approach to each individual need.