G-CSF prevents male infertility after chemotherapy.

描述（由申请方提供）：精原干细胞（SSC）是哺乳动物睾丸中维持精子发生的成体干细胞，对男性生育力至关重要。癌症治疗后SSC损失导致的男性不育症（例如，化疗和放疗）是许多癌症幸存者的生活质量问题。在这个建议中，我们将测试一种新的方法，以保持男性生育能力后，癌症治疗，采用注射细胞因子粒细胞集落刺激因子（G-CSF）。在目标1中，我们将证实G-CSF给药可预防化疗后精子发生和生育能力的丧失，并确定最佳治疗方案，以最大限度地发挥这些有益作用。在目标2中，我们将确定G-CSF治疗是否在SSC水平起作用，并将开始探讨其保护作用机制。如果发现有效，G-CSF治疗将完全消除对危险的侵入性干预的需要，因此，有可能显着改善癌症治疗后保留男性生育能力的临床范例。

DESCRIPTION (provided by applicant): Spermatogonial stem cells (SSCs) are adult stem cells in mammalian testes that maintain spermatogenesis and are essential for male fertility. Male infertility resulting from SSC loss after cancer treatments (e.g., chemotherapy and radiation) is a quality-of-life issue for many cancer survivors. In this proposal, we will test a nvel approach to preserving male fertility after cancer treatment that employs injections of the cytokine granulocyte colony-stimulating factor (G-CSF). In Aim 1, we will confirm that G-CSF administration prevents loss of spermatogenesis and fertility after chemotherapy and determine the optimal treatment regimen to maximize these beneficial effects. In Aim 2, we will determine whether G-CSF treatments act at the level of SSCs and will begin to address its protective mechanism of action. If found to be effective, G-CSF treatment would completely obviate the need for risky invasive interventions, and thus, has the potential to significantly improve the clinical paradigm for preserving male fertility after cancer treatment.