Single Cell Spatiotemporal and Functional Reporting using Magneto-Endosymbionts

描述(由申请人提供)：这项提案的目标是进一步开发我们的磁内共生(ME)技术，用于体内单细胞MRI检测(第一阶段)和功能报告(第二阶段)，从而为基于细胞的研究创造一个强大的翻译工具。MES是从磁性细菌中提取的，可以产生一种能够在体内追踪细胞的活性造影剂。磁共振成像是一种高度可翻译的技术，用于可视化移植细胞，因为它是 非侵入性，并提供极高的解剖分辨率。MES是一种独特的造影剂，因为它们具有巨大的遗传容量，可以用来编码体内的功能报告。MES对单细胞跟踪的有效性将在一个转移性脑癌模型中进行实验评估，我们以前曾使用该模型来演示超顺磁性氧化铁颗粒的单细胞MRI跟踪。在原位单细胞水平上提供对详细细胞事件的洞察的工具将促进肿瘤学研究、再生医学研究、个性化诊断和细胞疗法的临床实现。

DESCRIPTION (provided by applicant): The goal of this proposal is to further develop our magneto-endosymbiont (ME) technology for in vivo single cell MRI detection (Phase 1) and functional reporting (Phase 2) thus creating a robust translational tool for cell based research. MEs were derived from magnetic bacteria creating a living contrast agent that enables in vivo cell tracking. MRI is a highly translatable technique for visualizing implanted cells because it is non- invasive and provides extremely high anatomical resolution. MEs are a unique contrast agent because they have a large genetic capacity that can be leveraged to encode functional reporters, in vivo. The effectiveness of MEs for single cell tracking will be experimentally assessed in a metastatic brain cancer model, that we have previously used to demonstrate single cell MRI tracking with superparamagnetic iron oxide particles. Tools that provide insight into detailed cellular events at the single cell level in situ will advance oncology research, regenerative medicine research, personalized diagnostics and the clinical realization of cell therapies.