OTHER FUNCTION - Cell-free production of site-specificallyphosphorylated proteins

由于磷酸化蛋白在正常和异常细胞功能中起重要作用，因此迫切需要生产纯的、分析表征的磷酸化蛋白/多肽，用于设计用于捕获不同癌症的磷酸化特征的测定。磷酸化蛋白/多肽标准品可用作定量免疫测定的参考对照、用于产生磷酸化特异性抗体的免疫原，并且可将国家癌症研究所（NCI）65用于激酶抑制剂筛选以及蛋白-蛋白相互作用研究。

As phosphorylated proteins play a significant role in normal and abnormal cellular function, there is a critical need for the production of pure, analytically characterized phospho-proteins/polypeptides for use in assays designed to capture the phosphorylation signatures of different cancers. Phospho-protein/polypeptide standards can serve as reference controls for quantitative immunoassays, immunogens for generating phospho-specific antibodies, and can National Cancer Institute (NCI) 65 be utilized in kinase inhibitor screens as well as protein-protein interaction studies.