Behavioral Phenotyping

项目总结核心B：行为表型 行为表型分析核心将负责整合行为测试协议，并执行 支持成瘾系统神经遗传学中心的多维表型电池 （CSNA）.杰克逊实验室的小鼠神经行为表型分析设施（MNBF）是一个专门的 行为测试设备，优化用于在实验室小鼠中进行敏感性研究，其使命是提供 研究人员需要的资源和专业知识，进行全面和复杂的神经行为 试验.作为研究核心，MNBF凭借其现有的基础设施， 与成瘾的遗传基础相关的行为表型的表征。拟议 中心，MNBF将提供先进的行为表型分析能力和仪器， 接近鼠标资源，沿着专门的工作人员精通这些程序， 在各种近交系小鼠和异质的、高度多样化的远交系小鼠的行为测试中接受训练。 老鼠种群除了实验的执行，核心将支持复杂的解释， 行为，为生物库工作做出贡献，维护并提供测试方案，数据预 处理并通过LIMS系统传输到IGGC，以便在封闭环境中公开提供数据存储库 与整合遗传学和基因组学核心协调。对许多调查人员来说， 希望利用小鼠遗传学研究，特别是在异质和多样的小鼠种群中， 实验的规模和复杂性以及对专业知识的关键要求，特别是在 行为测试我们提出了三个具体目标：目标1：我们将验证相关的复杂行为测试 成瘾相关的行为;将它们整合到多维表型电池中。芯会 采用CSNA项目负责人的方案，并通过跨实验室检测验证证明 实用性、鲁棒性、可靠性以及将测试范例集成到多维测试中的能力 电池这一目标还将有助于展示工作人员执行复杂测试的熟练程度 电池，还有助于通知测定通量以及菌株间变异性。目标2：我们将执行 不同遗传、异质小鼠群体的行为表征（即，协作十字架， DO菌株），通过多维表型电池，以支持CSNA研究项目。 在试验验证后，根据在选定创始菌株中重现数据的能力（由 作为CSNA项目的领导者，我们将专注于应用训练有素的工作人员的专业知识来执行综合 在CSNA项目负责人的指导下，对这些小鼠群体进行电池测试。目标3：我们 为外部研究人员提供成瘾候选基因的深入表征， 基于中心的表型分析能力。核心将为这些调查人员提供设施， 专业知识和培训，以扩展候选基因的行为表征或提供全面的 新突变小鼠的行为表征，以评估与成瘾- 相关行为和相关共病，如焦虑、抑郁、认知和社会行为。在 此外，混淆主要行为终点解释的其他行为表型可能 也被评价。

PROJECT SUMMARY CORE B: BEHAVIORAL PHENOTYPING The Behavioral Phenotyping Core will be responsible for integrating behavioral testing protocols and executing multidimensional phenotyping batteries in support of the Center for Systems Neurogenetics of Addiction (CSNA). The Mouse Neurobehavioral Phenotyping Facility (MNBF) at The Jackson Laboratory is a dedicated behavioral testing facility optimized for conducting sensitive studies in laboratory mice with a mission to provide investigators the resources and expertise required to conduct comprehensive and complex neurobehavioral testing. As a research core, the MNBF is well-positioned with its existing infrastructure to support large-scale characterization of behavioral phenotypes relevant to the genetic underpinnings of addiction. In the proposed Center, the MNBF will provide advanced behavioral phenotyping capabilities and instrumentation in close proximity to mouse resources, along with dedicated staff proficient in conducting these procedures and highly trained in behavioral testing of mice of various inbred strains and the heterogeneous, highly diverse outbred mouse populations. In addition to the execution of experiments, the Core will support interpretation of complex behaviors, contribute to biobanking efforts, maintain and provide access to testing protocols, data pre- processing and transfer through the LIMS system to the IGGC for a publicly available data repository in close coordination with the Integrative Genetics and Genomics Core. A critical challenge for many investigators wishing to employ mouse genetics research, particularly in heterogeneous and diverse mouse populations, is the scale and complexity of experimentation as well as the critical requirements of expertise, particularly in behavioral testing. We propose three Specific Aims: Aim 1: We will validate complex behavioral tests relevant to addiction-related behaviors; integrating them into a multidimensional phenotyping battery. The Core will adopt protocols from the CSNA project leaders and demonstrate through cross-laboratory assay validation the utility, robustness, reliability, and the ability to integrate the testing paradigms into a multidimensional testing battery. This aim will also support the demonstration of the proficiency of staff to execute complex testing batteries and also help to inform assay throughput as well as inter-strain variability. Aim 2: We will perform the behavioral characterization of diverse genetic, heterogeneous mouse populations (i.e., Collaborative Cross, DO strains) through the multidimensional phenotyping batteries in support of the CSNA research projects. Following assay validation, as defined by the ability to reproduce data in select founder strains as published by the CSNA project leaders, we will focus on applying the expertise of trained staff to execute the integrated testing batteries on these mouse populations under the guidance of the CSNA project leaders. Aim 3: We will provide external investigators with in-depth characterization of addiction candidate genes using the broad- based phenotyping capabilities of the Center. The Core will provide these investigators with access to facilities, expertise, and training to extend behavioral characterization of candidate genes or provide comprehensive behavioral characterization of novel mutant mice to assess behavioral phenotypes relevant to both addiction- related behaviors and related co-morbidities such as anxiety, depression, cognition, and social behavior. In addition, other behavioral phenotypes that confound the interpretation of the primary behavioral endpoints may also be evaluated.