Biochemistry

项目摘要 该视觉研究核心（VRC）的总体目标是提供： ·获得个别R 01奖励范围以外的资源 ·获得单一实验室范围之外的技术专长 ·员工培训，以消除有效转化研究和合作的障碍 VRC支持的研究领域涵盖视网膜变性的分析和治疗， 发展障碍，青光眼和其他疾病，以及和一系列尖端的基本 科学倡议。我们已经实现了四个资源模块，继续自然演变， 这个研究小组如何协同工作，为15名研究人员提供21项NEI R 01奖。 生物化学模块从基因测序服务发展到真正的蛋白质组学（ultracen- 离心，凝胶定量）和代谢组学资源（专门的GC-MS，HPLC-MS，表面等离子体， Mon共振）更准确地反映了我们目前的研究优势。 具体来说，生物化学模块提供： ·基于Beckman XL-90的超离心 ·免疫印迹和杂交印迹的IR成像和定量 ·高容量Western印迹和ISH筛选; ·用于ECL和放射性检测的X射线胶片显影 ·将DNA构建体体内电穿孔到细胞中 ·小分子（类视色素、类胡萝卜素、脂褐质组分、脂肪酸）的HPLC-GC/MS ·利用SPR生物传感器技术定量蛋白质/配体相互作用 ·培训和全面的数据存档。

Project Summary The OVERALL aims of this Vision Research Core (VRC) are to provide: · access to resources outside the scope of individual R01 awards · access to technical expertise outside the scope a single laboratory · staff training to remove barriers to efficient translational research and collaboration The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as and a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 15 investigators holding 21 NEI R01 awards. The Biochemistry Module evolved from a gene sequencing service towards a true proteomics (ultracen- trifugation, gel quantitation) and metabolomics resource (specialized GC-MS, HPLC-MS, surface plas- mon resonance) more accurately reflecting our current research strengths. Specifically the Biochemistry Module provides: · Ultracentrifugation based on a workhorse Beckman XL-90 · IR imaging and quantitatiion of immunoblots and hybridization blots · High-capacity Western blotting and ISH screening; · X-ray film developing for ECL and radioactivity detection · Neonatal in vivo electroporation of DNA constructs into cells · HPLC-GC/MS of small molecules (retinoids, carotenoids, lipofuscin components, fatty acids) · Protein/ligand interaction quantitation with SPR biosensor technologies · training and comprehensive data archiving.