ULTRASOUND IMAGING CORE

摘要 核心D：非侵入性成像核心：该核心的目的是提供最先进的活体非侵入性成像方法，用于：(A)了解与母胎环境变化相关的心血管生理适应；以及(B)提供不依赖于动物牺牲的量化终点，因此可用于暂时表征母胎界面和胎儿心血管发育和功能的变化。这一核心利用了OHSU在使用非侵入性心血管成像来了解发育中胎儿的血管和心室生理学方面的优势。核心人员将由研究人员组成，他们开创了对比超声灌注成像的发展，并应用这些技术来评估健康和疾病中的微血管重构，并了解血流-功能关系。该项目的成像核心实验室的开发目的不仅是为了获得组织学或体外方法无法获得的关于心血管功能和微血管血流的独特信息，而且还为了创建一种经济高效的方法来评估大型动物模型中胎儿环境的影响，而不需要求助于安乐死。每个重大项目都纳入了对成像核心的利用，从而能够对核心服务进行可预测的分配。然而，OHSU的非侵入性心血管成像研究实验室的一个优势是能够适应合作用户的需求，以获得对分子表型和细胞生物学的独特理解。预计将根据个别研究计划的动态需求开发成像或新技术的更多应用。此外，心功能评估将是一项由核心提供的服务，使每个项目都将有一个完整的灌注和功能评估。每个项目都将利用核心。核心将应用领域中的新技术，因为它们可以为3个项目的应激模型中的胎儿心脏和胎盘的评估带来越来越多的动力。

Abstract Core D: Non-invasive Imaging Core: The purpose of this core is to provide state-of-the-art in vivo noninvasive imaging approaches for: (a) understanding the cardiovascular physiologic adaptations related to changes in maternal-fetal environment; and (b) to provide quantitative end-points that do not rely on animal sacrifice and, hence, can be used to temporally characterize changes in the maternal-fetal interface and fetal cardiovascular development and function. This core takes advantage of OHSU's strength in using noninvasive cardiovascular imaging for understanding vascular and ventricular physiology in the developing fetus. The core personnel will be composed of investigators who have pioneered the development of contrast ultrasound perfusion imaging and have applied these techniques to evaluate microvascular remodeling in health and disease and to understand flow-function relations. The imaging core lab for this project has been developed for the purpose of not only gaining unique information on cardiovascular function and microvascular flow that is not available by histology or ex vivo methods, but also to create a cost-efficient method for evaluating the impact of fetal environment in a large animal model without recourse to euthanasia. Utilization of the imaging core has been incorporated into each ofthe major projects, allowing for predictable allocation of core services. However, a strength of the non-invasive cardiovascular imaging research laboratory at OHSU has been the ability to adapt to the needs of collaborating users imaging technology in order to gain unique understanding of molecular phenotype and cell biology. It is anticipated that additional applications of imaging or new technologies will be developed according to the dynamic needs ofthe individual research programs. In addition, evaluation of cardiac function will be a service provided by the core so that each project will have an entire perfusion and functional evaluation. Each project will take advantage of the core. The core will apply the ever new technologies in the field as they become available to bring increasingly more power to the evaluation ofthe fetal heart and placenta in the stress models in the 3 projects.