Investigation into protein quality control pathways in Dictyostelium discoideum

项目摘要/摘要 蛋白质病是至少71种疾病的一类，其特征是蛋白质聚集物的积累。 蛋白质聚集体是由蛋白质内稳态的不平衡造成的，从而导致 错误折叠的蛋白质。生物医学研究中的一个主要问题是：细胞如何识别和处理 错误折叠的蛋白质？一个偶然的机会，我们发现模式生物盘基网柄菌正常 表达带有长多聚谷氨酰胺束的蛋白质，这会导致一类蛋白质病。我们最近做了 研究表明，网柄菌具有非凡的抵抗聚谷氨酰胺聚集性的能力 蛋白质已知在其他模式生物中聚集。在这里，我们建议研究由 网柄网柄菌抗多聚谷氨酰胺聚集及网柄网柄菌蛋白新特性的研究 质量控制网络。

Project Summary/Abstract Proteopathies are a class of at least 71 diseases characterized by the accumulation of protein aggregates. Protein aggregates are caused by an imbalance in protein homeostasis resulting in the accumulation of misfolded proteins. One major question in biomedical research is: How do cells recognize and deal with misfolded proteins? Serendipitously, we found that the model organism Dictyostelium discoideum normally expresses proteins with long polyglutamine tracts that cause one class of proteopathy. We have recently shown that Dictyostelium have an extraordinary ability to resist aggregation of a polyglutamine expanded protein know to aggregate in other model organisms. Here we propose to investigate mechanisms utilized by Dictyostelium to resist polyglutamine aggregation and explore novel aspects of the Dictyostelium protein quality control network.