Biological Fate and Biocompatability of Dendritic and Silica-Based Nanoconstructs

描述（由申请人提供）：该项目的总体目标是评估纳米材料的物理化学特性如何调节生物环境反应。在这个应用程序中，孔隙度，降解和弯曲二氧化硅纳米颗粒（snp）对细胞摄取，生物分布，药代动力学和毒性机制的影响将被检查。具体来说，我们将更详细地研究SNPs的细胞命运和细胞内转运机制，炎症反应是如何响应物理化学性质的变化而启动的，以及SNPs降解产物的生物学命运。提出了三个具体目标：1)1；在大小、孔隙度、表面粗糙度和功能以及生物降解性方面存在系统性差异的snp的合成和表征；2)研究表面特性如何影响蛋白质和细胞炎症介导机制；3)研究体内表面特性的慢性炎症介导机制。

DESCRIPTION (provided by applicant): The global goal of this project is to assess how physicochemical characteristics of nanomaterials modulate biological environmental responses. In this application the influence of porosity, degradation and curvature of silica nanoparticles (SNPs) on mechanisms of cellular uptake, biodistribution, pharmacokinetics and toxicity will be examined. Specifically, we will examine in more detail the cellular fate and mechanisms of intracellular transport of the SNPs, how inflammatory responses are initiated in response to the changes in the physicochemical properties, and what would be the biological fate of degradation products of SNPs. Three Specific Aims are proposed: 1) 1. Synthesis and characterization of SNPs with systematic differences in size, porosity, surface roughness and functionality, and biodegradability, 2) Investigate how surface properties affect protein and cellular inflammatory mediated mechanisms in vitro, 3) Investigate chronic inflammatory mediated mechanisms as a function of surface properties in vivo.