Dissolvable Hydrogel Dressing for the Treatment of Burns

 产品说明：本申请描述了水凝胶敷料的合成、表征和评价，该水凝胶敷料可溶解并可容易地从二度烧伤患者的伤口表面去除，而不会造成进一步的创伤。烧伤是最常见和最具破坏性的创伤形式之一。每年有超过30万人死于与火灾有关的烧伤，数百万人患有与烧伤有关的残疾和毁容，对幸存者及其家人造成心理、社会和经济影响。据报告，敷料移除是最疼痛的时间（烧伤本身之后），阿片类药物仍然是烧伤患者治疗的主要药物。在需要ICU/OR级别护理的典型损伤中，烧伤敷料更换的持续时间通常至少为60分钟，并伴有全身麻醉诱导，根据情况可能延长至3小时以上。目前，所有临床上批准的可用敷料都粘附在伤口表面上，使得敷料的每次更换都会导致身体表面外层上新形成的组织的创伤、延迟愈合以及受伤患者的巨大个人痛苦。本申请描述了硫醇封端的树枝化基元和双官能NHS活化的PEG，它们彼此反应以形成硫酯连接的水凝胶敷料，该水凝胶敷料随后可以通过经由硫醇-硫酯交换机制暴露于硫醇水溶液而溶解。拟议的实验将测试以下假设：基于水凝胶的可溶解烧伤敷料将提供感染屏障、促进伤口愈合，并且可以根据需要轻松移除。重要的是，它提供了初步数据，证明了可溶解水凝胶敷料原型的合成、表征和性能。这项为期四年的申请的具体目标是：目标1：合成和表征一系列水凝胶烧伤敷料;目标2：确定可溶解敷料皮下植入后的体外毒性和生物相容性以及体内相容性;目标3：评估可溶解敷料的体内功效。

 DESCRIPTION: This application describes the synthesis, characterization, and evaluation of a hydrogel dressing that dissolves and can be easily removed from the wound surface of a patient with second degree burns with no further trauma. Burns are one of the most common and devastating forms of trauma. Each year, more than 300,000 people die from fire-related burn injuries and millions suffer from burn-related disabilities and disfigurements with psychological, social, and economic effects on both the survivors and their families. Dressing removal is reported to be the time of most pain (after the burn itself) and opioids continue to be the mainstay of treatment for the burn patient. The duration of a burn dressing change in a typical injury requiring ICU/OR level care is often at least 60 minutes with induction of general anesthesia, which can extend to more than three hours depending on the case. At present, all clinically approved available dressings adhere to the wound surface so that each change of dressing leads to traumatization of newly formed tissues on the outer layer of the body's surface, delayed healing, and great personal suffering for the injured patient. The application describes a thiol- terminated dendron and a bifunctional NHS-activated PEG that react with each other to form a thioester-linked hydrogel dressing that can be subsequently dissolved by exposure of an aqueous thiol solution via a thiol- thiolester exchange mechanism. The proposed experiments will test the hypothesis that a hydrogel- based, dissolvable burn dressing will provide a barrier to infection, promote wound healing, and be easily removable on demand. Importantly, it presents preliminary data demonstrating the synthesis, characterization, and performance of a dissolvable hydrogel dressing prototype. The specific aims of this four- year application are: Aim 1: Synthesize and characterize a series of hydrogel burn dressings; Aim 2: Determine the in vitro toxicity and biocompatibility, as well as in vivo compatibility after subcutaneous implantation of the dissolvable dressing; and Aim 3: Evaluate the efficacy of the dissolvable dressing in vivo.