Imaging and Histology Core - "Mentoring in Cardiovascular Biology"

组织/细胞制剂的组织化学和免疫组织化学染色的细胞和分子成像 标记物使研究人员能够识别样本的生化成分和途径 亚细胞分辨率。科布雷成像和组织学核心在这一领域发挥了重要作用 为研究人员提供空间、设施、仪器、监督和活细胞显微镜培训 和组织标本(固定的和冰冻的)、激光捕获显微解剖、共聚焦成像和 在细胞和分子水平上研究心血管生物学的各种组织化学技术。 该核心的具体目标是：1)提供技术人员来协助和/或执行高度专业化 显微技术包括去卷积、共聚焦显微镜、3D重建、共定位、 比率成像。全内反射荧光(TIRF)、荧光共振能量转移(FRET)、 以及固定和活细胞样本的光漂白后荧光恢复(FRAP)；2)协助 组织样本的收集、处理和染色，包括标准和特殊染色，以及 免疫组织化学抗体标记；3)进行实验并协助调查人员 选择适当的特殊染色并解释组织学和免疫组织化学数据； 4)指导年轻的调查人员和学生，并就潜在的应用向高级调查人员提供建议 包括显微技术、组织学分析和免疫组织化学，以加强其研究 项目并提供开发翻译研究项目的途径；5)引导形象 组织学检查的分析方法。这一核心继续为科布雷和大学提供 研究人员，包括教师、博士后研究员和研究生，具有成像资源和 技术援助。无论研究人员是在细胞水平上研究心血管生物学， 组织，或整个动物，这个核心一直有助于我们的每个科布雷研究人员检查 不同心血管疾病状态的结构和/或组织学基础或确认。

Cell and molecular imaging of tissue/cell preparations stained with histochemical and immunohistochemical markers allows researchers to identify the biochemical components and pathways of specimens at subcellular resolution. The COBRE Imaging and Histology Core has been instrumental in this area and provides investigators with space, facilities, instrumentation, supervision, and training for microscopy of livecells and histological specimens (fixed and frozen), laser capture microdissection, confocal imaging, and a variety of histochemical techniques to investigate cardiovascular biology at the cellular and molecular level. The specific aims of this Core are: 1)To provide skilled personnel to assist and/or perform highly specialized microscopic techniques including deconvolution, confocal microscopy, 3D reconstruction, co-localization, ratio imaging. Total Internal Reflection Fluorescence (TIRF), fluorescent resonance energy transfer (FRET), and fluorescence recovery after photobleaching (FRAP) of both fixed and live cell samples; 2) To assist in the collection, processing and staining of histological samples, including standard and special stains, and immunohistochemical antibody labeling; 3) To conduct experiments and assist investigators with the selection of appropriate special stains as well as interpretation of histological and immunohistochemical data; 4) To mentor young investigators and students, and advise senior investigators, on the potential application of microscopic techniques, histological analysis and immunohistochemistry to enhance their research projects and provide avenues towards developing translational research projects; 5) To guide image analysis methodologies for histological examination. This Core continues to provide COBRE and University investigators, including faculty, postdoctoral fellows, and graduate students, with imaging recourses and technical assistance. Whether investigators are studying cardiovascular biology at the level ofthe cell, tissue, or whole animal, this core has been instrumental to each of our COBRE researchers in examining the structural and/or histological basis for, or confirmation of, different cardiovascular disease states.