Project 3: Linking maternal obesity and offspring cancer risks through integrati

项目摘要/摘要--项目3 肥胖已经成为一种全球流行病。仅在美国，根据疾控中心2008年的数据， 33.8%的成年人肥胖，68%的成年人肥胖或超重。夏威夷州有多个民族 其中夏威夷土著和太平洋岛民(NHPI)的肥胖率高达49.3%，是最高的 任何种族/民族的。母体肥胖的影响是跨代的，因为母体肥胖 增加了新生儿肥胖的可能性，而且新生儿肥胖很可能会持续到成年。 此外，肥胖与包括成人癌症在内的各种疾病有关。流行病学研究 提供了母体肥胖会导致成年后后代癌症风险增加的联系。 然而，由于人类研究中的伦理和实践原因，缺乏直接的证据。在这里，我们提出一种 多种族群体(亚洲人、夏威夷原住民、太平洋岛民和高加索人)的病例对照研究 研究母体肥胖和子代癌症风险之间的分子联系，通过整合 脐带血干细胞的转录组和甲基组。不明身份的脐带血组织将被收集 通过夏威夷生物库(HiBR)。具体地说，我们将选择造血干细胞，并进行 RNA-Seq转录组和甲基组实验。这些数据将与 转录组数据、甲基组数据以及整合的转录组和甲基组数据，来自 癌症基因组图谱。通过这种比较，我们希望找出癌症样的模块，这些模块标志着 肥胖母亲脐血干细胞与正常母亲脐血干细胞的差异 体重指数。这项研究有望为预防肥胖和癌症的发生提供有效的策略。 在孩子出生之前。它还将有助于提供补救战略，以解决悬而未决的健康差距 夏威夷州的问题。

PROJECT SUMMARY/ABSTRACT - Project 3 Obesity has become a global pandemic. In the Unites States alone, according the CDC data 2008, 33.8% adults were obese and 68% adults were obese or overweight. The State of Hawaii has multiple ethnic groups, in which Native Hawaiian and Pacific Islanders (NHPI) have a daunting obesity rate of 49.3%, highest of any racial/ethnic group. The effects of maternal obesity are trans-generational, in that maternal obesity increases the likelihood of neonatal obesity, and the neonatal obesity is likely to persist in the adulthood. Additionally, obesity is correlated with various diseases including cancers in adults. Epidemiological studies have provided the link that maternal obesity can lead to increased cancer risks in progenies in later adult life. However, due to ethical and practical reasons in human studies, direct evidence is lacking. Here we propose a case control study with multi-ethnic groups (Asian, Native Hawaiian and Pacific Islanders and Caucasians) to investigate the molecular links between maternal obesity and offspring cancer risks, through the integration of transcriptome and methylome of the cord blood stem cells. The de-identified cord blood tissue will be collected through the Hawaii Biorepository (HiBR). Specifically, we will select the hematopoietic stem cells, and perform RNA-Seq transcriptome and methylome experiments, respectively. These data will be compared to the transcriptome data, methylome data, as well as the integrated transcriptome and methylome data, from The Cancer Genome Atlas. Through such comparison, we hope to identify the cancer-like modules that signify the difference between cord blood stem cells associated with obese mothers vs. those of mothers with normal BMI. This study will hopefully help provide effective strategies to prevent obesity and cancers to occur even before the child is born. It will also help to provide remedial strategies to solve the outstanding health disparity issue in the State of Hawaii.