Core B: Animal Care and Germ-Free Mouse Core

项目概要/摘要-核心B。动物护理和无菌小鼠核心 拟议的密歇根小鼠代谢表型中心的动物护理和无菌小鼠核心 将在通过中心表型核心的动物流管理中发挥核心作用， 进口小鼠的采集至最终处置。核心主任和副主任都是DVM/PhD 兽医科学家和美国兽医病理学家学会的专家。联合主任是一名 兽医和美国实验动物医学院的兽医。的特定功能 动物护理核心包括协调老鼠进口、检疫和环境测试， 小鼠病原体组，根据 MMPC指南。此外，研究服务，如动手技术支持，内部临床和 诊断病理学，以及无菌技术和专业知识将是我们核心的独特组成部分。 将提供无菌C57 BL/6和Swiss-Webster小鼠以及几种基因工程菌株 MMPC调查员。此外，研究者可以从其他机构进口无菌小鼠或要求 新的无菌菌株的再衍生。可用于无菌和SPF小鼠的实验操作 包括常规治疗、样品收集和内部分析（血液、粪便、尿液等）、尸检 组织学，特殊组织化学染色和免疫组织化学，组织病理学解释，培训， 和实验设计咨询等等。动物护理和无菌小鼠核心的目标 为所有研究动物提供最高水平的健康和福祉以及兽医专业知识 在我们的USDA认证和AAALAC认证的设施。所有核心服务将在大学下进行 密歇根UCUCA批准的协议。所有SPF小鼠将饲养在Allentown通风架上。细菌- 将自由小鼠保持在隔离器或Tecniplast Isocage架中。屏障条件，包括笼 在垂直层流换流站中进行换流，严格遵守微隔离器技术，并使用 用于菌落隔离的隔离室将确保保持无病原体的动物。此外该 动物中心将监督技术支持，如给药、体重测定， 禁食、血糖水平、提供和监测特殊饮食以及将小鼠转移到ULAM饲养所和从ULAM饲养所转移小鼠 MMPC其他核心的实验室。

Project Summary / Abstract – Core B. Animal Care and Germ-Free Mouse Core The Animal Care and Germ-Free Mouse Core of the proposed Michigan Mouse Metabolic Phenotyping Center will play a central role in the management of animal flow through the Center's phenotyping cores from initial acquisition to final disposition of imported mice. Both the Core Director and Associate Director are DVM/PhD veterinary scientists and diplomates of the American College of Veterinary Pathologists. The Co-Director is a veterinarian and diplomate of the American College of Laboratory Animal Medicine. Specific functions of the Animal Care Core include the coordination of mouse importation, quarantine and environmental testing for a panel of murine pathogens, providing housing, husbandry and veterinary care of mice in accordance with the MMPC guidelines. In addition, research services such as hands-on technical support, in house clinical and diagnostic pathology, as well as germ-free technology and expertise will be unique components of our Core. Germ-free C57BL/6 and Swiss-Webster mice as well as several genetically engineered strains will be available to MMPC investigators. In addition, investigators may import germ-free mice from other institutions or request rederivation of new germ free strains. Experimental manipulations available for germ-free and SPF mice include routine treatments, sample collections and in-house analysis (blood, feces, urine, etc), necropsy, histology, special histochemical staining and immunohistochemistry, histopathologic interpretation, training, and experimental design consultation, among others. Goals of the Animal Care and Germ-Free Mouse Core are to provide the highest level of health and well-being as well as veterinary expertise for all research animals in our USDA-certified and AAALAC-accredited facilities. All Core services will be performed under University of Michigan UCUCA approved protocols. All SPF mice will be housed in Allentown ventilated racks. Germ- free mice will be maintained in isolators or Tecniplast Isocage racks. Barrier conditions including cage changing in vertical laminar flow changing stations, strict adherence to microisolator techniques, and use of isolated rooms for colony segregation will assure maintenance of pathogen free animals. In addition, the Animal Core will provide oversight for technical support such as dose administration, obtaining body weights, fasting, glucose levels, providing and monitoring special diets, and transfer of mice to and from ULAM housing to the laboratories of additional Cores of the MMPC.