Laminar Organization of Cortical Functional Connectivity in Monkeys

在需要注意力和工作记忆的任务中，前额叶和顶叶皮层表现出功能连接 以振荡锁相的形式。项目4将测试锁相依赖于 在源自第3层神经元的两个区域之间的直接连接上。通过监测电气 同时从皮层表面到白色物质的精细间隔活动，而猴子参与 在工作记忆（目标1）和注意（目标2）中，实验将检验标志性的特定预测， 函数连通性的最大值在第3层。通过监测已识别投射的电活动， 神经元，并通过检查阻断两个区域之间的投射的影响，实验将 测试直接投射对功能连接至关重要的具体预测（目标3）。结果 在项目4中获得的信息将与关于认知起源的中心假设直接相关。 精神分裂症的症状这一假说指出，认知缺陷的出现是因为大脑中的病理变化， 第3层锥体细胞干扰前额叶和顶叶皮质之间的功能连接。项目 1-3将集中在健康大脑和精神分裂症患者的第三层锥体细胞的特性上。项目5 将重点关注健康大脑和精神分裂症中的功能连接， 因果分析。项目4的独特贡献将是通过以下方式将这些研究领域联系起来： 建立第3层神经元在功能连接中的作用，如用连贯性和因果关系评估的 分析。 相关性（参见说明）： 本项目将建立第三层神经元在精神分裂症认知障碍中的作用。

In tasks requiring attention and working memory, prefrontal and parietal cortex exhibit functional connectivity in the form of oscillatory phase-locking. Project 4 will test the general hypothesis that phase-locking depends on direct connections between the two areas originating from neurons in layer 3. By monitoring electrical activity simultaneously at fine intervals from the cortical surface to the white matter, while monkeys engage in working memory (Aim 1) and attenfion (Aim 2), the experiments will test the speciflc predicfion that signs of funcfional connectivity are maximal in layer 3. By monitoring the electrical activity of identified projection neurons and by examining the impact of blocking projections between the two areas, the experiments will test the specific prediction that direct projections are critical for functional connectivity (Aim 3). Results obtained in Project 4 will have direct relevance to the Central Hypothesis regarding the origin of cognitive deflcits in schizophrenia. This hypothesis states that cognifive deficits arise because pathological changes in layer 3 pyramidal cells interfere with functional connectivity between prefrontal and parietal cortex. Projects 1-3 will focus on the properties of layer 3 pyramidal cells in the healthy brain and in schizophrenia. Project 5 will focus on funcfional connectivity in the healthy brain and in schizophrenia as measured with coherence and causality analyses. The unique contribufion of Project 4 will be to link these domains of inquiry by establishing the role of layer 3 neurons in functional connectivity as assessed with coherence and causality analyses. RELEVANCE (See instructions): This project will establish the role of layer 3 neurons in the cognitive deflcits in schizophrenia.