FUNCTIONAL MRI IN BEHAVING RHESUS MONKEY

该项目的长期目标是应用功能磁学 清醒行为猴SO的磁共振成像(FMRI)技术 强大的单单元记录技术可以与 通过核磁共振成像更全面地了解大脑功能。有能力 将变化与直接电子记录相关联将有助于 进一步阐明和扩展功能磁共振成像的有用性。功能磁共振成像将会有 补偿单个神经元的两个主要极限的潜力 记录猴子的行为--无法观察整个大脑 无法分辨皮质柱水平的细节和 帕奇斯。我们给恒河猴安装了颅骨植入物 与在单个神经元中用于刚性头部约束的相同 正在录音。在上一个资助年度的活动包括 建设基础设施和试点基本方法论 在4.7-T Bruker Avance-DRX中对猴子的研究。我们构建了一个 在4.7-T中支撑麻醉猴子的摇篮 扫描器，磁头牢固地固定在射频线圈内。为了 允许猴子将手臂保持在一个向前的位置 功能，我们已经设计和制造了一个半圆柱形的射频线圈 出色的射频均匀性和敏感度，为您腾出空间 在头下面。作为获得功能性磁共振的第一步 猴子的图像，使用粗体，我们检测到了血液动力学 直接干预对恒河猴大脑皮质的影响 (高碳酸血症)。我们选择在高碳酸血症的情况下控制血液流动， 而不是通过大脑活动，从而绕过可能的 通过麻醉，大脑之间的功能联系中断 活跃度和血流量。该项目将达到正式的 TRD项目将于明年开始，实验将继续进行 在清醒的猴子身上进行功能磁共振成像。

The long-term goal of this project is to apply Functional Magnetic Resonance imaging (fMRI) techniques to the awake behaving monkey so that powerful single unit recording techniques can be combined with more global views of brain function from MRI. The ability to correlate changes with direct electrical recordings will help to further clarify and extend the usefulness of fMRI. fMRI will have the potential to compensate for the two major limits of single-neuron recording in behaving monkeys - inability to survey the whole brain and inability to resolve detail at the level of cortical columns and patches. We have equipped a rhesus monkey with a cranial implant identical to those used for rigid head restraint during single-neuron recording. Activities during the preceding grant year have involved building basic apparatus and piloting fundamental methodology for the study of monkeys in the 4.7-T Bruker AVANCE-DRX. We constructed a cradle by which to support an anesthetized monkey in the 4.7-T scanner, with the head rigidly fixed inside the RF coil. In order to allow the monkey to hold the arms in a forward position during functional, we have designed and built a hemicylindrical RF coil with excellent RF homogeneity and sensitivity, which leaves free the space beneath the head. As a first step towards obtaining functional MR images of the monkey, using BOLD, we have detected a hemodynamic change in rhesus monkey cortex induced by a direct intervention (hypercapnia). We chose to manipulate blood flow with hypercapnia, rather than with brain activity, so as to circumvent the possible disruption, by anesthesia, of the functional linkage between brain activity and blood flow. This project will achieve a formal status of a TRD project begining next grant year, and experiments will continue towards carrying out fMRI in the awake monkey.