Analytical Chemistry, Cell Culture, NOX Delivery

核心A为项目研究人员提供了最先进的分析工具，技术和经验， 化学物质的表征和定量以及细胞分子的修饰， DNA、RNA、脂质、蛋白质和小分子代谢物。它还提供了良好表征的细胞系， 一氧化氮相关的DNA和蛋白质损伤剂向这些细胞的受控递送以确保质量控制 项目之间的可重复性。它作为一个服务，一个发展实验室， 一个程序的资源。个人研究人员根据需要在三个层次上使用这些设施： 实验室，监督分析，或作为训练有素的用户。此外，我们不断扩大和改善我们的 开发和获取促进研究计划的技术的能力。 具体目标每年都在变化，通常包括开发和改进项目相关的 分析方法，新硬件的评估，以及最近获得的或潜在有用的 软件在刚刚结束的一年里，这些都是与项目有关的发展和改进， 分析方法（例如，修饰酪氨酸的定量），新硬件的开发评估，以及 持续关注肽和蛋白质的靶向定量，例如，使用基于点击化学的 用于样品富集的衍生化。在成员调查中确定新的设备和专门知识需求 由行政核心进行，并与项目负责人进行一对一的讨论，并通过计划- 项目小组会议。

Core A provides Project researchers with state-of-the-art analytical tools, techniques and experience in the characterization and quantitation of chemical substances and modifications of cellular molecules such as DNA, RNA, lipids, proteins and small-molecule metabolites. It also provides well-characterized cell lines and controlled delivery of nitric-oxide-related DNA and protein damaging agents to these cells to insure quality-control and reproducibility among the Projects. It operates as a service, a developmental laboratory, and as a resource for the Program. Individual researchers use the facilities as needed at three levels: as a service lab, for supervised analyses, or as fully trained users. In addition, we continually expand and improve our capabilities with the development and acquisition of technologies that facilitate the research programs. Specific aims evolve yearly and typically include the development and improvement of Project-related analytical methods, evaluation of new hardware, and evaluation of recently-acquired or potentially useful software. For the year just ending, these were the development and improvement of project-related analytical methods (e.g., quantitation of modified tyrosines), exploitation evaluation of new hardware, and continued focus on targeted quantitation of peptides and proteins, e.g., using click-chemistry-based derivatization for sample enrichment. New equipment and expertise needs are identified in member surveys conducted by the Administrative Core and one-on-one discussions with Project leaders, and via Program- Project group meetings.