Community Crystallization Resource for Biological Macromolecules

X射线结晶学是用来在原子上显示生物大分子的主要方法 详细程度。晶体对于这些结构测定是必需的。高通量结晶 豪普特曼-伍德沃德研究所的筛选(HTS)实验室是一个社区资源，提供 以快速有效的方式提供结晶信息，从而使结构研究能够快速进行。 我们开发了两套1536化学鸡尾酒，旨在最大限度地提高结晶潜力和 结构和其他科学用生物大分子的化学溶解度信息 申请。在不到十分钟的时间里，液体处理系统将社区提供的样本与 化学鸡尾酒，装在一个紧凑的1536孔盘子里。我们想象着每周六次的实验 数周，并为用户提供即时访问图像。这包括内部开发的软件，以 查看和分析结果。我们使用了最先进的成像系统和飞秒脉冲激光来 检测晶体，包括用传统显微镜看不到的亚微米晶体。如果有 水晶，我们能够探测到它们。专家审查所有送往实验室的样本的结果，以 识别晶体，并将这一信息提供给调查人员。图形摘要报告提供 研究人员以易于理解的格式提供相关的化学溶解度和结晶信息。 HTS实验室的目标是为以下物质提供快速的溶解度和结晶信息 社区提供的样本，以实现快速的科学进程。我们的出版记录，并与 来自结构基因组学和科学界合作支持的出版物和PDB证词 我们在研究、开发和应用结晶分析方面有15年以上的历史，超过16,000种 数百名调查人员提供的样本。

X-ray crystallography is the predominant method used to visualize biological macromolecules at an atomic level of detail. Crystals are mandatory for these structure determinations. The High-Throughput Crystallization Screening (HTS) Laboratory at the Hauptman-Woodward Institute is a community resource that provides crystallization information in a rapid and efficient manner so that structural investigations can rapidly progress. We have developed two sets of 1536 chemical cocktails designed to maximize crystallization potential and chemical solubility information for biological macromolecules for both structural and other scientific applications. In less than ten minutes, liquid-handling systems combine the community-provided sample with chemical cocktails in a single, compact, 1536-well plate. We image the experiments at weekly intervals for six weeks and provide users with immediate access to images. This includes software developed in-house, to view and analyze the results. We employ a state-of-the-art imaging system with a femtosecond pulse laser to detect crystals, including submicron crystals that are too small to see using traditional microscopy. If there are crystals, we are able to detect them. An expert reviews the outcomes for all samples sent to the laboratory to identify crystals and provides this information to the investigators. A graphical summary report provides investigators with relational chemical solubility and crystallization information in readily interpretable formats. The goal of the HTS Laboratory is to provide a rapid turnaround of solubility and crystallization information for community-provided samples to enable rapid scientific process. Our publication records, and associated publications and PDB depositions from structural genomics and the scientific community collaborations support our 15+ year history in the research, development and application of crystallization assays to more than 16,000 samples provided by hundreds of investigators.