Modulation of nociception in the hornworm, Manduca sexta

项目摘要 伤害性感觉是对伤害性刺激的感官接受和处理，它被认为是 人类的痛苦。在出现伤害性刺激后，伤害性敏感化发生，在 人类会导致慢性疾病，如痛觉过敏症或痛觉过敏，在正常情况下 无害的刺激被认为是痛苦的。有人认为，伤害性敏感化 无脊椎动物可能是痛觉过敏症或痛觉过敏的模型，这个项目的目的是使用 以六纹夜蛾为模型的防御性攻击反应 痛觉过敏。作为一种无脊椎动物，六分枝杆菌是不受控制的，有一个简单的神经系统，可以 在体外以最少的曝气量维持数小时至数天，并具有可量化的防御能力 活体打击。此外，脊椎动物的疼痛信号分子似乎有同源的 在昆虫中的对应物。该项目的目标是： 1)根据伤害性敏感化的持续时间，来表征六纹夜蛾的伤害性敏化 反应和诱发敏化的刺激类型。 2)从电生理角度鉴定六纹夜蛾的感觉网络和伤害性感受器。 免疫组织化学，和 3)验证致敏和镇痛的分子机制。

Project Summary Nociception is the sensory reception and processing of noxious stimuli, which is perceived as pain in humans. Nociceptive sensitization occurs after presentation of a noxious stimulus, and in humans can result in chronic conditions such as allodynia or hyperalgesia, where normally innocuous stimuli are perceived as painful. It has been suggested that nociceptive sensitization in invertebrates may be models of allodynia or hyperalgesia, and this project aims to use the defensive strike response in the hornworm Manduca sexta as a model of allodynia and hyperalgesia. As an invertebrate, M. sexta is unregulated, has a simple nervous system that can be maintained with minimal aeration in vitro for hours to days, and has a quantifiable defensive strike in vivo. Moreover, vertebrate pain signaling molecules appear to have homologous counterparts in insects. The goals of this project are to: 1) Characterize nociceptive sensitization in M. sexta, in terms of the duration of the response and the types of stimuli inducing sensitization. 2) Identify the sensory network and nociceptors in M. sexta electrophysiologically and immunohistochemically, and 3) Verify the molecular mechanism of sensitization and analgesia.