Microscopy Core

项目摘要/摘要 第三阶段显微镜核心的目标是为Cobre调查人员提供成像工具和培训，以 使他们的心血管研究取得成功和可持续发展。核心将协助调查人员收集- ING显微图像将有助于发表他们的研究，获得进一步的资金，并分发- 发现新的见解，将他们的研究推向新的方向。核心将提供最先进的 在各种技术方面的设备和专业知识，包括组织学和组织化学分析，免疫组织化学 非组织化学和免疫细胞化学、原位杂交、光学显微镜、计算机辅助图像 分析和形态计量学，活细胞成像，活体旋转圆盘共聚焦和多光子显微镜， 透射电子显微镜，免疫金标记，以及先进的冷冻技术，如冷冻破裂- 真的和冷冻蚀刻。显微镜核心设备和工作人员集中在OMRF Cardio- 血管生物学研究计划，靠近Cobre实验室和心血管表型- ING CORE。这个中心位置促进了核心用户之间的高水平互动。核心将被分成- 由两位成像专家Florea Lupu博士和Courtney Griffin博士主持，三位经验丰富的 工作人员。在第一阶段和第二阶段取得成功的基础上，显微镜核心将扩大和加速 科布雷研究人员之间的研究效率和协作。

PROJECT SUMMARY/ABSTRACT The goal of the Phase III Microscopy Core is to provide COBRE investigators with imaging tools and training to make their cardiovascular research successful and sustainable. The Core will assist investigators with collect- ing microscopic images that will facilitate publication of their research, acquisition of further funding, and dis- covery of novel insights that push their research in new directions. The Core will provide state-of-the-art equipment and expertise in a variety of techniques, including histological and histochemical analyses, immu- nohistochemistry and immunocytochemistry, in-situ hybridization, photomicroscopy, computer-assisted image analysis and morphometry, live-cell imaging, intravital spinning-disk confocal and multiphoton microscopy, transmission electron microscopy, immunogold labeling, and advanced cryotechnologies such as freeze frac- ture and freeze etching. The Microscopy Core equipment and staff are centrally located in the OMRF Cardio- vascular Biology Research Program, in close proximity to the COBRE labs and the Cardiovascular Phenotyp- ing Core. This central location promotes a high level of interaction among Core users. The Core will be di- rected by two imaging experts, Drs. Florea Lupu and Courtney Griffin, with assistance from three experienced staff members. Building on its success during Phases I and II, the Microscopy Core will expand and accelerate research productivity and collaboration among COBRE investigators.