Chromatin and Gene Analysis Core

项目摘要-染色质和基因分析核心 染色质和基因分析核心提供技术和生物信息学基础设施，以优化 挖掘大量全基因组基因表达和染色质数据，这些数据是从 中心的工作。中心研究人员在全基因组染色质分析的几个方面处于领先地位， 包括在Brain中开创这些方法，这带来了几个技术挑战。我们已经优化了 几种下一代测序方法的方法，包括RNA-SEQ、CHIP-SEQ、ATAC-SEQ、原位 HIC和全基因组亚硫酸氢盐测序，以及其他，后者提供了 其他一系列独特的技术挑战。这些方法正越来越多地被用于研究个体 在给定的边缘脑区内的细胞类型，在小鼠和人类中都是如此。核心已经在以下方面建立了专业知识 将继续对获得的高度复杂的数据集进行分析，并将继续努力进一步改进现有工具。 我们中心产生的所有全基因组数据都由这个核心进行分析。同时，核心运行 以动物模型为核心，对已定义的动物模型进行常规的全基因组分析，从而提供 为个别项目中更具体和更复杂的措施奠定基础。事实上，每个项目 重点关注在小鼠抑郁症模型和人类中受到最严格调控的基因 抑郁症。此外，Core还试验了新的实验技术；例如，我们能够针对单个 体内单个脑区和细胞类型内对单个基因的染色质修饰，从而提供了一种 建立抑郁症表观遗传机制的无与伦比的证据水平。通过整合分析 在一个集中的核心内进行工作和常规的全基因组分析，我们确保严格控制数据 并便于比较四个单独项目的实验结果。这一整合还 这在财务上是合理的，因为我们专注于并最大限度地有效利用我们的专业知识。最后，核心是 负责与管理核心一起维护一个共享我们全基因组数据集的平台，并 在该中心的实验室以及科学界和公众中广泛使用分析工具。

PROJECT SUMMARY – CHROMATIN AND GENE ANALYSIS CORE The Chromatin and Gene Analysis Core provides the technical and bioinformatics infrastructure to optimally mine the large amount of genome-wide gene expression and chromatin data that are generated from the Center's work. Center investigators lead the field in several aspects of genome-wide chromatin analyses, including pioneering these approaches in brain, which offers several technical challenges. We have optimized methods for several next generation sequencing approaches, including RNA-seq, ChIP-seq, ATAC-seq, in situ HiC, and whole genome bisulfite sequencing, among others, for mouse and human brain, the latter offering an additional set of unique technical challenges. These approaches are being used increasingly to study individual cell types within a given limbic brain region, in both mouse and human. The Core has established expertise in analyzing the highly complex datasets obtained, and will continue work on further improving the tools available. All of the genome-wide data generated by our Center are analyzed by this Core. In parallel, the Core runs routine genome-wide assays on defined animal models, with the Animal Models Core, and thereby provides a foundation for the more specific and sophisticated measures in the individual Projects. Indeed, each Project focuses on genes that are among the most robustly regulated across mouse depression models and human depression. As well, the Core pilots novel experimental technologies; an example is our ability to target single chromatin modifications to a single gene within a single brain region and cell type in vivo, thus providing an unparalleled level of proof to establish epigenetic mechanisms of depression. By consolidating the analytical work and routine genome-wide analyses within a centralized Core, we ensure rigorous control over the data and facilitate comparisons of experimental findings across the four individual Projects. This consolidation also makes financial sense, since we concentrate and maximize efficient use of our expertise. Finally, the Core is responsible, with the Administrative Core, in maintaining a platform for sharing our genome-wide datasets and analytical tools across the Center's laboratories as well as with the scientific community and lay public at large.