Transgenic/Knockout Mouse Shared Resource

转基因/基因敲除小鼠共享资源：项目 摘要/摘要 基因工程小鼠模型(GEMM)已经成为癌症研究中不可或缺的工具， 它们的用途从研究肿瘤发生和发展的基本生物学到创造 用于评估新的治疗方法的基因准确的肿瘤模型。总的目标是 因此，转基因/基因敲除小鼠共享资源(TG-KO)是为了便于及时和经济地 弗吉尼亚联邦大学成员在癌症研究中生产和使用GEMM 梅西癌症中心(MCC)。这一目标将通过生产新的转基因材料来实现(包括 转基因、基因敲除、条件基因敲除和敲入小鼠)同时使用常规和CRISPR/Cas9- 以技术为基础，并通过提供全方位的支持服务来促进这些模型的使用， 包括繁殖群体管理、小鼠基因分型、小鼠品系重新衍生、胚胎和精子 冷冻保存和冷冻恢复，以及会诊。 TG-KO由Jolene Windle博士执导，她有近30年的制作和 在癌症研究中使用GEMM，并得到2.5名额外FTE的支持，他们在 GEMM的创造、GEMM生产和筛选中涉及的分子方法和育种群体 管理层。TG-KO是一个联合管理的(MCC和VCU)资源，距离步行很近 MCC成员实验室。TG-KO设施包括受限进入的实验室和动物看守所 分子医学研究大楼隔膜的空间，以及普通分子 生物实验室位于楼下一层，所有设施齐全，可提供服务。TG-KO 没有固定的工作时间；但工作人员的一部分通常从上午8：30开始 周一至周五，直到下午6点或更晚。可以随时通过电子邮件联系到所有工作人员，以及 他们通常会尽快回复调查人员的电子邮件，通常是在正常工作时间以外。 对TG-KO提供的服务进行跟踪和收费。其他支持由 机构来源和癌症中心支持赠款，允许TG-KO在以下位置提供服务 价格明显低于商业供应商收取的价格。TG提供的服务- KO通过提供实际得不到或成本高昂的必要服务，为MCC研究增加价值 通过替代供应商，包括其他非营利机构，令人望而却步。通过结合In- 凭借国内的专业知识和服务以及极具竞争力的价格，TG-KO极大地促进了GEMM的使用 MCC成员在其基础和转化型癌症研究方面的努力。2015财年，TG-KO为 MCC会员18名，相当于用户基数的67%和全部充值的67%。

TRANSGENIC/KNOCKOUT MOUSE SHARED RESOURCE: PROJECT SUMMARY/ABSTRACT Genetically engineered mouse models (GEMMs) have become indispensable tools in cancer research, with their utility ranging from studies of the basic biology of tumorigenesis and progression to the creation of genetically accurate tumor models for the evaluation of novel therapeutic approaches. The overall objective of the Transgenic/Knockout Mouse Shared Resource (TG-KO) is therefore to facilitate timely and cost-effective production and use of GEMMs in cancer research by members of the Virginia Commonwealth University (VCU) Massey Cancer Center (MCC). This objective will be met by producing novel GEMMs (including transgenic, knockout, conditional knockout and knock-in mice) using both conventional and CRISPR/Cas9- based technologies and by providing a full range of support services to facilitate the use of these models, including breeding colony management, mouse genotyping, mouse line rederivation, embryo and sperm cryopreservation and cryorecovery, and consultation. The TG-KO is directed by Jolene Windle, PhD, who has nearly 30 years of experience in the production and use of GEMMs in cancer research, and is supported by 2.5 additional FTEs who bring added expertise in GEMM creation, molecular methods involved in GEMM production and screening, and breeding colony management. The TG-KO is a jointly managed (MCC and VCU) resource that is within close walking distance of MCC member laboratories. The TG-KO facility includes restricted-access laboratory and animal holding space in the barrier vivarium in the Molecular Medicine Research Building, as well as general molecular biology laboratory space on the floor below, all of which is fully equipped for the services provided. The TG-KO does not have fixed hours of operation; however, a subset of staff members is generally present from 8:30 AM until 6:00 PM or later, Monday through Friday. All members of the staff can be readily reached by email, and they typically respond to emails from investigators as soon as possible, often outside of regular work hours. The services provided by the TG-KO are tracked and charged back. Additional support is provided by institutional sources and the Cancer Center Support Grant, which allows the TG-KO to offer its services at rates that are significantly lower than those charged by commercial vendors. The services provided by the TG- KO add value for MCC research by providing necessary services that are not practically obtained or are cost prohibitive through alternative vendors, including other non-profit institutions. Through the combination of in- house expertise and services and highly competitive rates, the TG-KO greatly facilitates the use of GEMMs by MCC members in their basic and translational cancer research efforts. In CY2015 TG-KO provided services to 18 MCC members, corresponding to 64% of the user base and 67% of all recharges.