Analytical, Metabolic, Instrumentation Core

分析、代谢、仪器(AMI)中心核心和蛋白质组子核心 急性心肌梗死核心是对研究之前的两个核心进行重组和重组的结果 2007年在肝病中心(RCLD)。新成立的Core将现有的共享 仪器基础和分析资源服务，这两个都持续增长和 基本上是从那时起。此外，核心建立了蛋白质组学分核心，它在成立之初是一个 以实验室为基础的实体，中心成员是其被召唤服务的唯一用户基础。 随后，RCLD的领导导致南加州大学医学院和教务长的坚定承诺 办公室，确保资本资金用于新的、最先进的仪器设备和空间翻新。因此， 自那以后，亚核已经成长为大学范围的蛋白质组学核心。然而，核心，位于一层楼 在中央核心之下，继续作为次核心向中心成员提供优先访问/服务。这个 以下是中央核心和蛋白质组学次核心的目标。 中央核心：中央核心的目标是提供以下资源和服务： 1)广泛的(目前为26个)维护良好和急需的普通和高级基础设施， 共享接入使用的最先进设备。2)功能齐全、功能强大的高效液相系统，配备 光电二极管阵列、荧光、电化学和放射性检测器，提供15种分析方法 和准备好的分离。3)新安装的用于建模-仿真应用程序的计算机工作站 拥有越来越多的软件工具，目前支持DILIsym、MITOsym、SimPop和MatLab包。 4)协助--为调查员及其工作人员评价、选择和最佳利用 核心的资源和服务基础。5)培训-针对手中不断发展的用户群- 关于核心的资源和服务的运作。 蛋白质组学分核心：该分核心的目标是提供最先进的基于质谱学的 优先向中心成员提供蛋白质组学服务，包括：1)蛋白质测序和鉴定 从凝胶带和溶液中蛋白质的复杂混合物。2)翻译后的分析与确定 蛋白质的修饰。3)定量和定性的蛋白质组学图谱。4)建议/协助 实验策略-与蛋白质纯化和蛋白质组学项目相关的设计。

ABSTRACT– Analytical, Metabolic, Instrumentation (AMI) Central Core and Proteomics Subcore The AMI Core resulted from reorganization and restructuring of two of the previous cores of the Research Center for Liver Diseases (RCLD) in 2007. The newly established Core pooled the existing shared instrumentation base and the analytical resources-services, both of which have grown continuously and substantially since. In addition, the Core established a Proteomics Subcore, which at its inception was a laboratory-based entity, with Center Members being its exclusive user base it was called to serve. Subsequently, RCLD's leadership led to a strong commitment from USC School of Medicine and the Provost's office, securing capital funding for new, state-of-the-art instrumentation and space renovation. Thus, the Subcore has grown since to become a university-wide Proteomics Core. However, the Core, located a floor below the Central Core, continues providing priority access/service to Center Members as a Subcore. The following are the goals of the Central Core and Proteomics Subcore. Central Core: The aims of the Central Core are to provide the following resources and services: 1) An extensive (currently 26) base of well maintained and critically needed common as well as advanced, state-of-the-art equipment for shared access-use. 2) A versatile and powerful HPLC system equipped with photodiode array, fluorescence, electrochemical and radioactivity detectors, offering a menu of 15 analytical and preparative separations. 3) A newly installed computer workstation for modeling-simulation applications with a growing set of software tools, currently supporting DILIsym, MITOsym, SimPop and MATLAB packages. 4) Assisting-advising investigators and their personnel in evaluation, selection and optimal utilization of the Core's base of resources and services. 5) Training-orientation of a constantly evolving base of users in hands- on operation of the core's resources and services. Proteomics Subcore: The aims of the Subcore are to provides state-of-the-art mass spectrometry-based proteomics services on a priority basis to Center Members, including: 1) Protein sequencing and identification from gel bands and complex mixture of proteins in solutions. 2) Analysis and determination of post-translational modification in proteins. 3) Quantitative and qualitative proteomic profiling. 4) Advising/assisting with experimental strategies-designs related to protein purification and proteomics projects.