Optimization of Cancer Therapy guided by oxygen, pH, thiol and other parametric images

电子顺磁共振（EPR）氧图像（EPROI）已被证明可以提供定量 动物肿瘤的局部pO2图像，包括同系小鼠、大鼠和兔中的几种肿瘤。的 parent grant已经证明了50%肿瘤控制剂量的统计学显着锐化， 添加由小于10 μ m的EPROI体素的分数（HF 10）提供的信息。具体目标 对于当前授权周期：1）确定来自所述授权周期的局部氧信息之间的关系。 EPROI和哌莫硝唑滞留、缺氧蛋白诱导和单次活检缺氧分数。（二） 小鼠肿瘤治疗后氧像TCD50测定及预测疗效 HF10和其他EPROI肿瘤统计数据，以及3）使用来自EPROI的HF10对 缺氧与IMRT，以确定EPROI对小鼠肿瘤治愈的影响。这项工作将验证 定量氧成像在优化辐射输送和刺激氧气的发展 用于治疗人类肿瘤的成像。对研究专家职位的资助将加强 通过引入共形射束辐射成功实施母基金所需的技术 pH和氧化还原状态指导的调强放射治疗的方案和开发技术， 小鼠肿瘤这种支持将为在赠款周期之间获得资金提供充足的时间。

Electron Paramagnetic Resonance (EPR) Oxygen Images (EPROI) have been shown to provide quantitative localized pO2 images of animal tumors including several in syngeneic mice, and in rats, and rabbits. The parent grant has demonstrated statistically significant sharpening of the 50% tumor control dose with the addition of the information provided by the fraction of EPROI voxels less than 10 torr (HF10). Specific aims for the current grant cycle: 1) Determining the relation between the localized oxygen information from the EPROI and pimonidazole retention, induction of hypoxia proteins and single biopsy hypoxic fraction. 2) Treatment of murine tumors to TCD50 after oxygen images and determining the predictive effectiveness of the HF10 and other EPROI tumor statistics and 3) Using the HF10 from EPROI to dose paint regions of hypoxia with IMRT to determine the impact of the EPROI on cure of mouse tumors. This work will validate quantitative oxygen imaging in optimizing radiation delivery and stimulate the development of oxygen imaging for treatment of human tumors. The funding of the Research Specialist position will enhance the technology required for the success of the parent grant through introduction of conformal beam radiation protocol and develop technology for pH and redox status guided intensity modulated radiation therapy in mouse tumors. Such support will provide sufficient time to secure funding between grant cycles.