Shared Resource: Cell-Based Assay Screening Service

CORE：基于细胞的检测筛选服务共享资源（细胞和体内生物学组） 项目摘要 癌症的复杂性和异质性需要基于细胞的技术平台， 研究人员发现关键基因，途径，相互作用和修饰，推动癌症的发展， 以设计有效的个性化治疗方法。基于细胞的检测筛选服务（C-BASS） 共享资源为DLDCC成员提供了尖端技术的独特组合， 先进的仪器和基因组资源C-BASS包含单基因的基本元素 全基因组筛选分析，包括全基因组短发夹RNA（shRNA）文库，BiFC标记的 （双分子荧光互补）cDNA文库和多个最先进的自动化机器人 用于文库操作、用于表型分析的高通量筛选和数据处理的仪器 屏幕的基础设施。共享资源由托马斯韦斯特布鲁克博士和刘丹博士指导， 在开发和实施全基因组筛查技术方面拥有广泛的专业知识。这种组合 的专业知识和资源极大地促进了DLDCC研究人员在他们的癌症研究工作。具体 由C-BASS共享资源提供的服务包括（1）执行全基因组或亚基因组测序， 大规模BiFC和RNAi筛选，（2）利用单个cDNA和shRNA载体，（3）大规模自动化 cDNA和shRNA文库的操作和制备，（4）自动化哺乳动物细胞转染， 慢病毒生产，（5）使用自动显微镜或流式细胞术的高通量基于细胞的测定， （6）数据分析、存储和管理。在过去的几个月里，C-BASS还增加了基因组- 使用CRISPR/Cas9系统的编辑服务。CRISPR/Cas9不断扩展的功能， 包括基因靶向、基因组修饰和转录调节，有可能产生巨大 为荒漠化、土地退化和气候变化协调委员会成员增加价值。通过将这些资源容纳在一个单一的、有凝聚力的设施中，C-BASS能够 癌症中心研究人员采用各种基因组和遗传平台，这些平台通常成本高， 劳动力成本过高，或者对个体研究人员来说根本不可行。

CORE: Cell-Based Assay Screening Services Shared Resource (Cell & in vivo Biology Group) PROJECT SUMMARY The complexity and heterogeneity of cancer requires cell-based technological platforms that enable researchers to uncover key genes, pathways, interactions, and modifications that drive cancer development in order to devise effective and personalized therapeutics. The Cell-Based Assay Screening Service (C-BASS) Shared Resource provides DLDCC members with a unique combination of cutting-edge technologies, advanced instrumentation, and genomic resources. C-BASS houses essential elements for single-gene analyses to whole-genome screens including genome-wide short-hairpin RNA (shRNA) libraries, BiFC-tagged (Bi-molecular Fluorescence Complementation) cDNA libraries and multiple state-of-the-art automated robotic instruments for library manipulation, high-throughput screening for phenotypic analyses, and data processing infrastructure for screens. The Shared Resource is directed Drs. Thomas Westbrook and Dan Liu who have extensive expertise in developing and implementing technologies for genome-wide screens. This combination of expertise and resources greatly facilitates DLDCC investigators in their cancer research efforts. Specific services provided by the C-BASS Shared Resource include (1) performing whole-genome or sub-genome scale BiFC and RNAi screens, (2) utilizing individual cDNA and shRNA vectors, (3) large-scale automated manipulation and preparation of cDNA and shRNA libraries, (4) automated mammalian cell transfection and lentivirus production, (5) high-throughput cell-based assays using automated microscopy or flow cytometry, and (6) data analysis, storage, and management. In the past few months, C-BASS has also added genome- editing services using the CRISPR/Cas9 system. The ever-expanding functionalities of CRISPR/Cas9, which include gene targeting, genome modification, and transcription modulation, promises to be of tremendous added value to DLDCC members. By housing these resources in a single, cohesive facility, C-BASS enables Cancer Center Investigators to employ a variety of genomic and genetic platforms that are often cost- and labor-prohibitive, or simply not feasible for individual researchers.