A conditional and rapid cell ablation technology in zebrafish

项目总结 靶向细胞消融是研究细胞功能、组织再生、 发育过程和疾病发病机制。有几种遗传方法可用于细胞消融， 但消融过程相对缓慢，至少需要几个小时。在以下情况下，这可能是一个不利因素 在发育过程中或在 生理学。这项建议测试了一种新的遗传细胞消融技术的可行性 用于调节斑马鱼快速细胞消融的哺乳动物。初步数据表明，该系统运行良好。 用培养的斑马鱼神经元进行体外培养。这些研究将对优化条件进行有效和快速的 体内细胞消融，在原肠胚胎到游动幼虫。

PROJECT SUMMARY Targeted cell ablation is a powerful approach for investigating cellular functions, tissue regeneration, developmental processes and disease pathogenesis. Several genetic methods are available for cell ablation, but the ablation process is relatively slow, requiring at least several hours. This can be a disadvantage when examining relatively rapid cellular events (ranging from seconds to minutes) during development or in physiology. This proposal tests the feasibility of a novel genetic cell ablation technology established in mammals for mediating rapid cell ablation in zebrafish. Preliminary data indicate that the system works very well in vitro with cultured zebrafish neurons. These studies will optimize the conditions for effective and rapid cell ablation in vivo, in gastrulating embryos to swimming larvae.